Vestigial is required during late-stage muscle differentiation in Drosophila melanogaster embryos

Hua Deng, John B. Bell, Andrew J. Simmonds

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The somatic muscles of Drosophila develop in a complex pattern that is repeated in each embryonic hemi-segment. During early development, progenitor cells fuse to form a syncytial muscle, which further differentiates via expression of muscle-specific factors that induce specific responses to external signals to regulate late-stage processes such as migration and attachment. Initial communication between somatic muscles and the epidermal tendon cells is critical for both of these processes. However, later establishment of attachments between longitudinal muscles at the segmental borders is largely independent of the muscle-epidermal attachment signals, and relatively little is known about how this event is regulated. Using a combination of null mutations and a truncated version of Sd that binds Vg but not DNA, we show that Vestigial (Vg) is required in ventral longitudinal muscles to induce formation of stable intermuscular attachments. In several muscles, this activity may be independent of Sd. Furthermore, the cell-specific differentiation events induced by Vg in two cells fated to form attachments are coordinated by Drosophila epidermal growth factor signaling. Thus, Vg is a key factor to induce specific changes in ventral longitudinal muscles 1-4 identity and is required for these cells to be competent to form stable intermuscular attachments with each other.

Original languageEnglish (US)
Pages (from-to)3304-3316
Number of pages13
JournalMolecular biology of the cell
Volume21
Issue number19
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

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Drosophila melanogaster
Embryonic Structures
Muscles
Drosophila
Epidermal Growth Factor
Tendons
Cell Differentiation
Stem Cells
Communication
Mutation
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Vestigial is required during late-stage muscle differentiation in Drosophila melanogaster embryos. / Deng, Hua; Bell, John B.; Simmonds, Andrew J.

In: Molecular biology of the cell, Vol. 21, No. 19, 01.10.2010, p. 3304-3316.

Research output: Contribution to journalArticle

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