Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes

Anju Sreelatha, Terry L. Bennett, Emily M. Carpinone, Kevin M. O'Brien, Kamyron D. Jordan, Dara L. Burdette, Kim Orth, Vincent J. Starai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Vesicle fusion governs many important biological processes, and imbalances in the regulation of membrane fusion can lead to a variety of diseases such as diabetes and neurological disorders. Here we show that the Vibrio parahaemolyticus effector protein VopQ is a potent inhibitor of membrane fusion based on an in vitro yeast vacuole fusion model. Previously, we demonstrated that VopQ binds to the Vo domain of the conserved V-type H+-ATPase (V-ATPase) found on acidic compartments such as the yeast vacuole. VopQ forms a nonspecific, voltage-gated membrane channel of 18 A˚ resulting in neutralization of these compartments. We now present data showing that VopQ inhibits yeast vacuole fusion. Furthermore, we identified a unique mutation in VopQ that delineates its two functions, deacidification and inhibition of membrane fusion. The use of VopQ as a membrane fusion inhibitor in this manner now provides convincing evidence that vacuole fusion occurs independently of luminal acidification in vitro.

Original languageEnglish (US)
Pages (from-to)100-105
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number1
DOIs
StatePublished - Jan 6 2015

Fingerprint

Vacuolar Proton-Translocating ATPases
Membrane Fusion
Proton-Translocating ATPases
Vibrio
Vacuoles
Membranes
Yeasts
Proteins
Vibrio parahaemolyticus
Biological Phenomena
Nervous System Diseases
Ion Channels
Mutation
In Vitro Techniques

Keywords

  • SNARE
  • Vesicle fusion
  • Vibrio parahaemolyticus
  • vp1680
  • Yeast vacuole

ASJC Scopus subject areas

  • General

Cite this

Sreelatha, A., Bennett, T. L., Carpinone, E. M., O'Brien, K. M., Jordan, K. D., Burdette, D. L., ... Starai, V. J. (2015). Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes. Proceedings of the National Academy of Sciences of the United States of America, 112(1), 100-105. https://doi.org/10.1073/pnas.1413764111

Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes. / Sreelatha, Anju; Bennett, Terry L.; Carpinone, Emily M.; O'Brien, Kevin M.; Jordan, Kamyron D.; Burdette, Dara L.; Orth, Kim; Starai, Vincent J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 1, 06.01.2015, p. 100-105.

Research output: Contribution to journalArticle

Sreelatha, Anju ; Bennett, Terry L. ; Carpinone, Emily M. ; O'Brien, Kevin M. ; Jordan, Kamyron D. ; Burdette, Dara L. ; Orth, Kim ; Starai, Vincent J. / Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 1. pp. 100-105.
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