Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors

Suneeta Chimalapati; Alexander E. Lafrance; Luming Chen; Kim Orth

doi:10.1002/cpmc.131

Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors

Suneeta Chimalapati, Alexander E. Lafrance, Luming Chen, Kim Orth

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.

Original language	English (US)
Article number	e131
Journal	Current Protocols in Microbiology
Volume	59
Issue number	1
DOIs	https://doi.org/10.1002/cpmc.131
State	Published - Dec 2020

Keywords

cytotoxicity
genetic manipulation
growth
immunofluorescence
invasion
motility
secretion

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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10.1002/cpmc.131

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abstract = "Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.",

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author = "Suneeta Chimalapati and Lafrance, {Alexander E.} and Luming Chen and Kim Orth",

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AU - Lafrance, Alexander E.

AU - Chen, Luming

AU - Orth, Kim

PY - 2020/12

Y1 - 2020/12

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AB - Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.

KW - cytotoxicity

KW - genetic manipulation

KW - growth

KW - immunofluorescence

KW - invasion

KW - motility

KW - secretion

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Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors

Abstract

Keywords

ASJC Scopus subject areas

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