Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors

Suneeta Chimalapati, Alexander E. Lafrance, Luming Chen, Kim Orth

Research output: Contribution to journalArticlepeer-review

Abstract

Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.

Original languageEnglish (US)
Article numbere131
JournalCurrent Protocols in Microbiology
Volume59
Issue number1
DOIs
StatePublished - Dec 2020

Keywords

  • cytotoxicity
  • genetic manipulation
  • growth
  • immunofluorescence
  • invasion
  • motility
  • secretion

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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