@article{ad4a95cd306540e3b075ac5361f552c1,
title = "Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors",
abstract = "Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.",
keywords = "cytotoxicity, genetic manipulation, growth, immunofluorescence, invasion, motility, secretion",
author = "Suneeta Chimalapati and Lafrance, {Alexander E.} and Luming Chen and Kim Orth",
note = "Funding Information: The authors thank current and past members of the Orth laboratory for their valuable contributions in designing these protocols. This work was funded by grants from the National Institutes of Health: R01 GM115188 (KO), 5 T32 AI007520‐20 (AEL), 5 T32 GM008014 (LC), and from the Once Upon a Time Foundation and the Welch Foundation I‐1561. Dr. Kim Orth is a Burroughs Welcome Investigator, a Beckman Young Investigator, and a W. W. Caruth, Jr., Biomedical Scholar with an Earl A. Forsythe Chair in Biomedical Science. Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals LLC",
year = "2020",
month = dec,
doi = "10.1002/cpmc.131",
language = "English (US)",
volume = "59",
journal = "Current Protocols in Microbiology",
issn = "1934-8525",
publisher = "John Wiley and Sons Inc.",
number = "1",
}