Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxins

Thomas Calder, Lisa N. Kinch, Jessie Fernandez, Dor Salomon, Nick V. Grishin, Kim Orth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is a Gram-negative halophilic bacterium and one of the leading causes of food-borne gastroenteritis. Its genome harbors two Type III Secretion Systems (T3SS1 and T3SS2), but only T3SS2 is required for enterotoxicity seen in animal models. Effector proteins secreted from T3SS2 have been previously shown to promote colonization of the intestinal epithelium, invasion of host cells, and destruction of the epithelial monolayer. In this study, we identify VPA1380, a T3SS2 effector protein that is toxic when expressed in yeast. Bioinformatic analyses revealed that VPA1380 is highly similar to the inositol hexakisphosphate (IP6)-inducible cysteine protease domains of several large bacterial toxins. Mutations in conserved catalytic residues and residues in the putative IP6-binding pocket abolished toxicity in yeast. Furthermore, VPA1380 was not toxic in IP6 deficient yeast cells. Therefore, our findings suggest that VPA1380 is a cysteine protease that requires IP6 as an activator.

Original languageEnglish (US)
Article numbere104387
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 6 2014

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bacterial toxins
Bacterial Toxins
Vibrio
Cysteine Proteases
cysteine proteinases
Yeast
Yeasts
Poisons
yeasts
Vibrio parahaemolyticus
type III secretion system
Phytic Acid
gastroenteritis
Gastroenteritis
Intestinal Mucosa
Bioinformatics
intestinal mucosa
Ports and harbors
Computational Biology
Gram-Negative Bacteria

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxins. / Calder, Thomas; Kinch, Lisa N.; Fernandez, Jessie; Salomon, Dor; Grishin, Nick V.; Orth, Kim.

In: PLoS One, Vol. 9, No. 8, e104387, 06.08.2014.

Research output: Contribution to journalArticle

Calder, Thomas ; Kinch, Lisa N. ; Fernandez, Jessie ; Salomon, Dor ; Grishin, Nick V. ; Orth, Kim. / Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxins. In: PLoS One. 2014 ; Vol. 9, No. 8.
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