Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

Metasebya Solomon, Brian R. White, Ralph E. Nothdruft, Walter Akers, Gail Sudlow, Adam T. Eggebrecht, Samuel Achilefu, Joseph P. Culver

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.

Original languageEnglish (US)
Pages (from-to)3267-3277
Number of pages11
JournalBiomedical Optics Express
Volume2
Issue number12
DOIs
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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