Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration

Zhuo Gan, Liya Ding, Christoph J. Burckhardt, Jason Lowery, Assaf Zaritsky, Karlyndsay Sitterley, Andressa Mota, Nancy Costigliola, Colby G. Starker, Daniel F. Voytas, Jessica Tytell, Robert D. Goldman, Gaudenz Danuser

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Increased expression of vimentin intermediate filaments (VIFs) enhances directed cell migration, but the mechanism behind VIFs’ effect on motility is not understood. VIFs interact with microtubules, whose organization contributes to polarity maintenance in migrating cells. Here, we characterize the dynamic coordination of VIF and microtubule networks in wounded monolayers of retinal pigment epithelial cells. By genome editing, we fluorescently labeled endogenous vimentin and α-tubulin, and we developed computational image analysis to delineate architecture and interactions of the two networks. Our results show that VIFs assemble an ultrastructural copy of the previously polarized microtubule network. Because the VIF network is long-lived compared to the microtubule network, VIFs template future microtubule growth along previous microtubule tracks, thus providing a feedback mechanism that maintains cell polarity. VIF knockdown prevents cells from polarizing and migrating properly during wound healing. We suggest that VIFs’ templating function establishes a memory in microtubule organization that enhances persistence in cell polarization in general and migration in particular.

Original languageEnglish (US)
Pages (from-to)252-263.e8
JournalCell Systems
Volume3
Issue number3
DOIs
StatePublished - Sep 28 2016

Fingerprint

Cell Polarity
Intermediate Filaments
Vimentin
Microtubules
Retinal Pigments
Tubulin
Wound Healing
Cell Movement
Epithelial Cells
Maintenance

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration. / Gan, Zhuo; Ding, Liya; Burckhardt, Christoph J.; Lowery, Jason; Zaritsky, Assaf; Sitterley, Karlyndsay; Mota, Andressa; Costigliola, Nancy; Starker, Colby G.; Voytas, Daniel F.; Tytell, Jessica; Goldman, Robert D.; Danuser, Gaudenz.

In: Cell Systems, Vol. 3, No. 3, 28.09.2016, p. 252-263.e8.

Research output: Contribution to journalArticle

Gan, Z, Ding, L, Burckhardt, CJ, Lowery, J, Zaritsky, A, Sitterley, K, Mota, A, Costigliola, N, Starker, CG, Voytas, DF, Tytell, J, Goldman, RD & Danuser, G 2016, 'Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration', Cell Systems, vol. 3, no. 3, pp. 252-263.e8. https://doi.org/10.1016/j.cels.2016.08.007
Gan, Zhuo ; Ding, Liya ; Burckhardt, Christoph J. ; Lowery, Jason ; Zaritsky, Assaf ; Sitterley, Karlyndsay ; Mota, Andressa ; Costigliola, Nancy ; Starker, Colby G. ; Voytas, Daniel F. ; Tytell, Jessica ; Goldman, Robert D. ; Danuser, Gaudenz. / Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration. In: Cell Systems. 2016 ; Vol. 3, No. 3. pp. 252-263.e8.
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