Viorio VopQ induces Pu-kmase-independent autophagy and antagonizes phagocytosis

Dara L. Burdette, Joachim Seemann, Kim Orth

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is a Gram-negative bacterium responsible for gastroenteritis acquired from the consumption of contaminated shellfish. This bacterium harbours two type III secretion systems, one on each chromosome. The type III secretion system on chromosome I induces cell death by a temporally controlled sequence of events that is caspaseindependent and first involves induction of autophagy, followed by cellular rounding, and finally cellular lysis. VopQ is a type III secreted effector that is necessary for the induction of autophagy as mutant strains lacking VopQ are attenuated in their ability to induce autophagy during infection. VopQ is sufficient to induce rapid autophagy as demonstrated by microinjection of recombinant VopQ into GFP-LC3 HeLa cells. Our results demonstrate that VopQ is both necessary and sufficient for induction of autophagy during V. parahaemolyticus-med iated cell death and this effect is independent of phosphatidylinositol-3kinases but requires Atg5. Furthermore, induction of VopQ-mediated autophagy prevents recruitment of the necessary cellular machinery required for phagocytosis of V. parahaemolyticus during infection. These data provide important insights into the mechanism used by V. parahaemolyticus to cause disease.

Original languageEnglish (US)
Pages (from-to)639-649
Number of pages11
JournalMolecular Microbiology
Volume73
Issue number4
DOIs
StatePublished - Aug 2009

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Autophagy
Phagocytosis
Vibrio parahaemolyticus
Cell Death
Chromosomes
Shellfish
Gastroenteritis
Microinjections
Phosphatidylinositols
Infection
Gram-Negative Bacteria
HeLa Cells
Bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Viorio VopQ induces Pu-kmase-independent autophagy and antagonizes phagocytosis. / Burdette, Dara L.; Seemann, Joachim; Orth, Kim.

In: Molecular Microbiology, Vol. 73, No. 4, 08.2009, p. 639-649.

Research output: Contribution to journalArticle

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