Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins

Cheng Ming Chiang, Mart Ustav, Arne Stenlund, Thau F. Ho, Thomas R. Broker, Louise T. Chow

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

We have shown that E1 and E2 proteins of human papillomavirus type 11 (HPV-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (BPV-1). Unexpectedly, matched or even mixed combinations of E1 and E2 proteins from HPV-11 or BPV-1 replicated either ori in human, monkey, and rodent cell lines of epithelial or fibroblastic lineage, albeit with varied efficiencies. Either set of viral proteins was also able to initiate replication of ori-containing plasmids from many other human and animal papillomaviruses. Thus the interactions among the cis elements and trans factors of papillomaviruses are more conserved than expected from the other members of the papovavirus family, simian virus 40 and polyomavirus, for which large tumor antigen does not replicate a heterologous ori in either permissive or nonpermissive cells. We infer that the stringent species and tissue specificities observed for papillomaviruses in vivo are not entirely due to direct restrictions on viral DNA replication. Rather, transcriptional control of viral gene expression must play a dominant role.

Original languageEnglish (US)
Pages (from-to)5799-5803
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number13
StatePublished - Jul 1 1992

Fingerprint

Bovine papillomavirus 1
Species Specificity
Polyomavirus
Organ Specificity
Replication Origin
Simian virus 40
Viral Genes
Viral DNA
Viral Proteins
Neoplasm Antigens
DNA Replication
Haplorhini
Rodentia
Proteins
Plasmids
Gene Expression
Cell Line
Human papillomavirus type 11 E2 protein
Human papillomavirus type 11 E1 protein

Keywords

  • Eukaryotic DNA replication
  • Human papillomaviruses
  • Replication factors

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins. / Chiang, Cheng Ming; Ustav, Mart; Stenlund, Arne; Ho, Thau F.; Broker, Thomas R.; Chow, Louise T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 13, 01.07.1992, p. 5799-5803.

Research output: Contribution to journalArticle

Chiang, Cheng Ming ; Ustav, Mart ; Stenlund, Arne ; Ho, Thau F. ; Broker, Thomas R. ; Chow, Louise T. / Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 13. pp. 5799-5803.
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