Viral transduction of trkA into cultured nodose and spinal motor neurons conveys NGF responsiveness

Hong Xu, Howard Federoff, Johnna Maragos, Luis F. Parada, John A. Kessler

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The trkA gene encodes the tyrosine kinase receptor that transduces the NGF signal. We have used a defective herpes simplex virus-1 (HSV-1) vector containing a full-length rat trkA sequence (pHSVtrkA) to express NGF receptors in primary cultures of neonatal nodose neurons and embryonic spinal motor neurons which are normally unresponsive to NGF. Transduction of rat neonatal nodose ganglion neurons and spinal motor neurons with pHSVtrkA resulted in expression of functional NGF receptors. Stimulation of these receptors by NGF resulted in the survival and the morphologic and biochemical differentiation of these neurons. These observations indicate that nodose and spinal motor neurons can express the downstream signal transduction apparatus necessary to mediate the survival and differentiating effects mediated by ligand binding to trkA and that transduction with pHSVtrkA can effectively convert NGF nonresponsive neurons into responsive ones.

Original languageEnglish (US)
Pages (from-to)152-161
Number of pages10
JournalDevelopmental Biology
Volume163
Issue number1
DOIs
StatePublished - 1994

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Motor Neurons
Nerve Growth Factor
Nerve Growth Factor Receptors
Neurons
Nodose Ganglion
Defective Viruses
Human Herpesvirus 1
Receptor Protein-Tyrosine Kinases
Signal Transduction
Ligands
Genes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Viral transduction of trkA into cultured nodose and spinal motor neurons conveys NGF responsiveness. / Xu, Hong; Federoff, Howard; Maragos, Johnna; Parada, Luis F.; Kessler, John A.

In: Developmental Biology, Vol. 163, No. 1, 1994, p. 152-161.

Research output: Contribution to journalArticle

Xu, Hong ; Federoff, Howard ; Maragos, Johnna ; Parada, Luis F. ; Kessler, John A. / Viral transduction of trkA into cultured nodose and spinal motor neurons conveys NGF responsiveness. In: Developmental Biology. 1994 ; Vol. 163, No. 1. pp. 152-161.
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