Viral vector production: Adenovirus

Julius W. Kim, Ramin A. Morshed, J. Robert Kane, Brenda Auffinger, Jian Qiao, Maciej S. Lesniak

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Adenoviral vectors have proven to be valuable resources in the development of novel therapies aimed at targeting pathological conditions of the central nervous system, including Alzheimer’s disease and neoplastic brain lesions. Not only can some genetically engineered adenoviral vectors achieve remarkably efficient and specific gene delivery to target cells, but they also may act as anticancer agents by selectively replicating within cancer cells. Due to the great interest in using adenoviral vectors for various purposes, the need for a comprehensive protocol for viral vector production is especially apparent. Here, we describe the process of generating an adenoviral vector in its entirety, including the more complex process of adenoviral fiber modification to restrict viral tropism in order to achieve more efficient and specific gene delivery.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages115-130
Number of pages16
Volume1382
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1382
ISSN (Print)1064-3745

Keywords

  • Adenoviral gene therapy
  • Adenovirus
  • Adenovirus fiber modification
  • Adenovirus tropism
  • Viral vector production

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kim, J. W., Morshed, R. A., Kane, J. R., Auffinger, B., Qiao, J., & Lesniak, M. S. (2016). Viral vector production: Adenovirus. In Methods in Molecular Biology (Vol. 1382, pp. 115-130). (Methods in Molecular Biology; Vol. 1382). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3271-9_9