Abstract
We present a novel slit scanning confocal microscope with a CCD camera image sensor and a virtual slit aperture for descanning that can be adjusted during post-processing. A very efficient data structure and mathematical criteria for aligning the virtual aperture guarantee the ease of use. We further introduce a method to reduce the anisotropic lateral resolution of slit scanning microscopes. System performance is evaluated against a spinning disk confocal microscope on identical specimens. The virtual slit scanning microscope works as the spinning disk type and outperforms on thick specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 499-505 |
| Number of pages | 7 |
| Journal | Histochemistry and Cell Biology |
| Volume | 128 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2007 |
Keywords
- Confocal microscopy
- Microscopy
- Scanning microscopy
ASJC Scopus subject areas
- Histology
- Molecular Biology
- Medical Laboratory Technology
- Cell Biology