Virtual slit scanning microscopy

Reto Fiolka, Andreas Stemmer, Yury Belyaev

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We present a novel slit scanning confocal microscope with a CCD camera image sensor and a virtual slit aperture for descanning that can be adjusted during post-processing. A very efficient data structure and mathematical criteria for aligning the virtual aperture guarantee the ease of use. We further introduce a method to reduce the anisotropic lateral resolution of slit scanning microscopes. System performance is evaluated against a spinning disk confocal microscope on identical specimens. The virtual slit scanning microscope works as the spinning disk type and outperforms on thick specimens.

Original languageEnglish (US)
Pages (from-to)499-505
Number of pages7
JournalHistochemistry and Cell Biology
Volume128
Issue number6
DOIs
StatePublished - Dec 2007

Keywords

  • Confocal microscopy
  • Microscopy
  • Scanning microscopy

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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