Virulence and stress-related periplasmic protein (VisP) in bacterial/host associations

Cristiano G. Moreira, Carmen M. Herrera, Brittany D. Needham, Christopher T. Parker, Stephen J. Libby, Ferric C. Fang, M. Stephen Trent, Vanessa Sperandio

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Gram-negative bacteria have an outer membrane containing LPS. LPS is constituted of an oligosaccharide portion and a lipid-A moiety that embeds this molecule within the outer membrane. LPS is a pathogen-associated molecular pattern, and several pathogens modify their lipid-A as a stealth strategy to avoid recognition by the innate immune system and gain resistance to host factors that disrupt the bacterial cell envelope. An essential feature of Salmonella enterica Typhimurium pathogenesis is its ability to replicate within vacuoles in professional macrophages. S. Typhimurium modifies its lipid-A by hydroxylation by the Fe2+/a-ketoglutarate-dependent dioxygenase enzyme (LpxO). Here, we show that a periplasmic protein of the bacterial oligonucleotide/ oligosaccharide-binding fold family, herein named virulence and stress-related periplasmic protein (VisP), on binding to the sugarmoiety of peptidoglycan interacts with LpxO. This interaction inhibits LpxO function, leading to decreased LpxO-dependent lipid-A modifications and increasing resistance to stressors within the vacuole environment during intramacrophage replication promoting systemic disease. Consequently, δvisP is avirulent in systemic murine infections, where VisP acts through LpxO. Several Gram-negative pathogens harbor both VisP and LpxO, suggesting that this VisP-LpxOmechanismof lipid-Amodifications has broader implications in bacterial pathogenesis. Bacterial species devoid of LpxO (e.g., Escherichia coli) have no lipid-A phenotypes associated with the lack of VisP; however, VisP also controls LpxO-independent phenotypes. VisP and LpxO act independently in the S. Typhimurium murine colitis model, with both mutants being attenuated for diverging reasons; δvisP is less resistant to cationic antimicrobial peptides, whereas δlpxO is deficient for epithelial cell invasion. VisP converges bacterial cell wall homeostasis, stress responses, and pathogenicity.

Original languageEnglish (US)
Pages (from-to)1470-1475
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number4
DOIs
StatePublished - Jan 22 2013

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Periplasmic Proteins
Virulence
Lipid A
Vacuoles
Oligosaccharides
Periplasmic Binding Proteins
Antimicrobial Cationic Peptides
Phenotype
Dioxygenases
Salmonella enterica
Membranes
Peptidoglycan
Salmonella typhimurium
Colitis
Hydroxylation
Gram-Negative Bacteria
Oligonucleotides
Cell Wall
Immune System
Homeostasis

ASJC Scopus subject areas

  • General

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Virulence and stress-related periplasmic protein (VisP) in bacterial/host associations. / Moreira, Cristiano G.; Herrera, Carmen M.; Needham, Brittany D.; Parker, Christopher T.; Libby, Stephen J.; Fang, Ferric C.; Trent, M. Stephen; Sperandio, Vanessa.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 4, 22.01.2013, p. 1470-1475.

Research output: Contribution to journalArticle

Moreira, Cristiano G. ; Herrera, Carmen M. ; Needham, Brittany D. ; Parker, Christopher T. ; Libby, Stephen J. ; Fang, Ferric C. ; Trent, M. Stephen ; Sperandio, Vanessa. / Virulence and stress-related periplasmic protein (VisP) in bacterial/host associations. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 4. pp. 1470-1475.
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