Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration

Christopher A. Lopez, Brittany M. Miller, Fabian Rivera-Chávez, Eric M. Velazquez, Mariana X. Byndloss, Alfredo Chávez-Arroyo, Kristen L. Lokken, Renée M. Tsolis, Sebastian E. Winter, Andreas J. Bäumler

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Citrobacter rodentium uses a type III secretion system (T3SS) to induce colonic crypt hyperplasia in mice, thereby gaining an edge during its competition with the gutmicrobiota through an unknown mechanism. Here, we sho wthat by triggering colonic crypt hyperplasia, the C. rodentium T3SS induced an excessive expansion of undifferentiated Ki67-positive epithelial cells, which increased oxygenation of themucosal surface and drove an aerobic C. rodentium expansion in the colon. Treatment ofmice with the g-secretase inhibitor dibenzazepine to diminish Notch-driven colonic crypt hyperplasia curtailed the fitness advantage conferred by aerobic respiration during C. rodentium infection. We conclude that C. rodentium uses its T3SS to induce histopathological lesions that generate an intestinal microenvironment in which growth of the pathogen is fueled by aerobic respiration.

Original languageEnglish (US)
Pages (from-to)1249-1253
Number of pages5
JournalScience
Volume353
Issue number6305
DOIs
StatePublished - Sep 16 2016

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Citrobacter rodentium
Virulence Factors
Respiration
Hyperplasia
Amyloid Precursor Protein Secretases
Colon
Epithelial Cells
Growth
Infection

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Lopez, C. A., Miller, B. M., Rivera-Chávez, F., Velazquez, E. M., Byndloss, M. X., Chávez-Arroyo, A., ... Bäumler, A. J. (2016). Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration. Science, 353(6305), 1249-1253. https://doi.org/10.1126/science.aag3042

Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration. / Lopez, Christopher A.; Miller, Brittany M.; Rivera-Chávez, Fabian; Velazquez, Eric M.; Byndloss, Mariana X.; Chávez-Arroyo, Alfredo; Lokken, Kristen L.; Tsolis, Renée M.; Winter, Sebastian E.; Bäumler, Andreas J.

In: Science, Vol. 353, No. 6305, 16.09.2016, p. 1249-1253.

Research output: Contribution to journalArticle

Lopez, CA, Miller, BM, Rivera-Chávez, F, Velazquez, EM, Byndloss, MX, Chávez-Arroyo, A, Lokken, KL, Tsolis, RM, Winter, SE & Bäumler, AJ 2016, 'Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration', Science, vol. 353, no. 6305, pp. 1249-1253. https://doi.org/10.1126/science.aag3042
Lopez CA, Miller BM, Rivera-Chávez F, Velazquez EM, Byndloss MX, Chávez-Arroyo A et al. Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration. Science. 2016 Sep 16;353(6305):1249-1253. https://doi.org/10.1126/science.aag3042
Lopez, Christopher A. ; Miller, Brittany M. ; Rivera-Chávez, Fabian ; Velazquez, Eric M. ; Byndloss, Mariana X. ; Chávez-Arroyo, Alfredo ; Lokken, Kristen L. ; Tsolis, Renée M. ; Winter, Sebastian E. ; Bäumler, Andreas J. / Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration. In: Science. 2016 ; Vol. 353, No. 6305. pp. 1249-1253.
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