Virulence properties of atypical EPEC strains

J. S. Pelayo, I. C A Scaletsky, M. Z. Pedroso, V. Sperandio, J. A. Girón, G. Frankel, L. R. Trabulsi

Research output: Contribution to journalArticle

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Abstract

Virulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive. Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for α-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h. Three strains produced enterohaemolysin on blood agar. None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin β, whereas all the strains from serotype O55:H7 expressed intimin γ. The strains belonging to sero-group O111 expressed a non-typable intimin. The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalJournal of Medical Microbiology
Volume48
Issue number1
StatePublished - 1999

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Enteropathogenic Escherichia coli
Virulence
Hemolysin Proteins
Vero Cells
DNA Probes
Gentamicins
Transmission Electron Microscopy
HeLa Cells
Agar
Actins
Cultured Cells
Diarrhea
Staining and Labeling
Escherichia coli
Rabbits
Antibodies
Serogroup
enterohemolysin

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Pelayo, J. S., Scaletsky, I. C. A., Pedroso, M. Z., Sperandio, V., Girón, J. A., Frankel, G., & Trabulsi, L. R. (1999). Virulence properties of atypical EPEC strains. Journal of Medical Microbiology, 48(1), 41-49.

Virulence properties of atypical EPEC strains. / Pelayo, J. S.; Scaletsky, I. C A; Pedroso, M. Z.; Sperandio, V.; Girón, J. A.; Frankel, G.; Trabulsi, L. R.

In: Journal of Medical Microbiology, Vol. 48, No. 1, 1999, p. 41-49.

Research output: Contribution to journalArticle

Pelayo, JS, Scaletsky, ICA, Pedroso, MZ, Sperandio, V, Girón, JA, Frankel, G & Trabulsi, LR 1999, 'Virulence properties of atypical EPEC strains', Journal of Medical Microbiology, vol. 48, no. 1, pp. 41-49.
Pelayo JS, Scaletsky ICA, Pedroso MZ, Sperandio V, Girón JA, Frankel G et al. Virulence properties of atypical EPEC strains. Journal of Medical Microbiology. 1999;48(1):41-49.
Pelayo, J. S. ; Scaletsky, I. C A ; Pedroso, M. Z. ; Sperandio, V. ; Girón, J. A. ; Frankel, G. ; Trabulsi, L. R. / Virulence properties of atypical EPEC strains. In: Journal of Medical Microbiology. 1999 ; Vol. 48, No. 1. pp. 41-49.
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