Virulent Treponema pallidum, lipoprotein, and synthetic lipopeptides induce CCR5 on human monocytes and enhance their susceptibility to infection by human immunodeficiency virus type 1

Timothy J. Sellati, David A. Wilkinson, Jeanne S. Sheffield, Richard A. Koup, Justin D. Radolf, Michael V. Norgard

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Treponema pallidum, its membrane lipoproteins, and synthetic lipoprotein analogues (lipopeptides) were each examined to determine whether they induced CCR5 expression on human peripheral blood mononuclear cells (PBMC). Reverse transcription-polymerase chain reaction for CCR5 gene transcripts, macrophage inflammatory protein (MIP)-1β binding assays, and flow cytometry revealed that either T. pallidum, a representative treponemal lipoprotein, or a corresponding synthetic lipopeptide induced CCR5 on CD14 monocytes but not on CD3 lymphocytes. CXCR4, the coreceptor for T cell-tropic strains of human immunodeficiency virus type 1 (HIV-1), was not induced on PBMC by treponemes or by lipoproteins or lipopeptides. Consistent with these findings, T. pallidum, lipoprotein, and synthetic lipopeptide all promoted the entry of a macrophage-tropic, but not a T cell-tropic, strain of HIV-1 into monocytes. These combined results imply that T. pallidum and its constituent lipoproteins likely induce the expression of CCR5 on macrophages in syphilitic lesions, thereby enhancing transmission of macrophage-tropic HIV- 1.

Original languageEnglish (US)
Pages (from-to)283-293
Number of pages11
JournalJournal of Infectious Diseases
Volume181
Issue number1
DOIs
StatePublished - 2000

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Lipopeptides
Treponema pallidum
Lipoproteins
HIV-1
Monocytes
Infection
Macrophages
Blood Cells
T-Lymphocytes
Macrophage Inflammatory Proteins
Protein Binding
Reverse Transcription
Flow Cytometry
Lymphocytes
Polymerase Chain Reaction
Membranes
Genes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Virulent Treponema pallidum, lipoprotein, and synthetic lipopeptides induce CCR5 on human monocytes and enhance their susceptibility to infection by human immunodeficiency virus type 1. / Sellati, Timothy J.; Wilkinson, David A.; Sheffield, Jeanne S.; Koup, Richard A.; Radolf, Justin D.; Norgard, Michael V.

In: Journal of Infectious Diseases, Vol. 181, No. 1, 2000, p. 283-293.

Research output: Contribution to journalArticle

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