TY - JOUR
T1 - Virulent Treponema pallidum, lipoprotein, and synthetic lipopeptides induce CCR5 on human monocytes and enhance their susceptibility to infection by human immunodeficiency virus type 1
AU - Sellati, Timothy J.
AU - Wilkinson, David A.
AU - Sheffield, Jeanne S.
AU - Koup, Richard A.
AU - Radolf, Justin D.
AU - Norgard, Michael V.
N1 - Funding Information:
Financial support: NIH (AI-16692, AI-26756, AI-38894, AI-35522, AI-42397, and AI-42630) and Robert A. Welch Foundation (I-0940). R.A.K. was an Elizabeth Glaser Scientist of the Pediatric AIDS Foundation. T.J.S. was supported by a National Research Service Award postdoctoral fellowship (AI-09973) from NIH and by a research fellowship award from the Arthritis Foundation.
PY - 2000
Y1 - 2000
N2 - Treponema pallidum, its membrane lipoproteins, and synthetic lipoprotein analogues (lipopeptides) were each examined to determine whether they induced CCR5 expression on human peripheral blood mononuclear cells (PBMC). Reverse transcription-polymerase chain reaction for CCR5 gene transcripts, macrophage inflammatory protein (MIP)-1β binding assays, and flow cytometry revealed that either T. pallidum, a representative treponemal lipoprotein, or a corresponding synthetic lipopeptide induced CCR5 on CD14 monocytes but not on CD3 lymphocytes. CXCR4, the coreceptor for T cell-tropic strains of human immunodeficiency virus type 1 (HIV-1), was not induced on PBMC by treponemes or by lipoproteins or lipopeptides. Consistent with these findings, T. pallidum, lipoprotein, and synthetic lipopeptide all promoted the entry of a macrophage-tropic, but not a T cell-tropic, strain of HIV-1 into monocytes. These combined results imply that T. pallidum and its constituent lipoproteins likely induce the expression of CCR5 on macrophages in syphilitic lesions, thereby enhancing transmission of macrophage-tropic HIV- 1.
AB - Treponema pallidum, its membrane lipoproteins, and synthetic lipoprotein analogues (lipopeptides) were each examined to determine whether they induced CCR5 expression on human peripheral blood mononuclear cells (PBMC). Reverse transcription-polymerase chain reaction for CCR5 gene transcripts, macrophage inflammatory protein (MIP)-1β binding assays, and flow cytometry revealed that either T. pallidum, a representative treponemal lipoprotein, or a corresponding synthetic lipopeptide induced CCR5 on CD14 monocytes but not on CD3 lymphocytes. CXCR4, the coreceptor for T cell-tropic strains of human immunodeficiency virus type 1 (HIV-1), was not induced on PBMC by treponemes or by lipoproteins or lipopeptides. Consistent with these findings, T. pallidum, lipoprotein, and synthetic lipopeptide all promoted the entry of a macrophage-tropic, but not a T cell-tropic, strain of HIV-1 into monocytes. These combined results imply that T. pallidum and its constituent lipoproteins likely induce the expression of CCR5 on macrophages in syphilitic lesions, thereby enhancing transmission of macrophage-tropic HIV- 1.
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U2 - 10.1086/315209
DO - 10.1086/315209
M3 - Article
C2 - 10608777
AN - SCOPUS:0033983314
SN - 0022-1899
VL - 181
SP - 283
EP - 293
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -