Viscoelastic properties of phonosurgical biomaterials at phonatory frequencies

Miwako Kimura, Ted Mau, Roger W. Chan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objectives/Hypothesis: The purpose of this study was to examine the functional biomechanical properties of several injectable biomaterials currently or potentially used for vocal fold augmentation. Study Design: Rheometric investigation of phonosurgical materials in vitro. Methods: Linear viscoelastic shear properties of 3% bovine collagen (atelocollagen), micronized Allo-Derm (Cymetra; LifeCell Corp., Branchburg, NJ), calcium hydroxylapatite (CaHA) (Radiesse; BioForm Medical, San Mateo, CA), and 2.4% cross-linked hyaluronic acid (HA) gel (Juvéderm; Allergan, Inc., Irvine, CA) were quantified as functions of frequency covering the phonatory range, and compared to those of the human vocal fold cover. Measurements of elastic shear modulus (G′) and dynamic viscosity (η′) were made at up to 250 Hz with a controlled-strain simple-shear rheometer. Linear least-squares regression was conducted to curve-fit log G′ and log η′ versus log frequency, and statistical analysis was performed with one-way analysis of variance. Results: Radiesse and Cymetra were found to be the stiffest and the most viscous materials, followed by Juvéderm and atelocollagen. There were significant differences in the magnitudes of G′ and η′ among the phonosurgical materials and the normal human vocal fold cover (p < .001), whereas there was no significant difference in the frequency dependence of G′ and η′ among the materials. Post hoc Tukey tests revealed significant differences (p < .05) in pairwise comparisons of the magnitudes of G′ and η′ among all materials and the vocal fold cover. Conclusions: These findings suggested that although these biomaterials may be injected lateral to the lamina propria for the treatment of glottic insufficiency, none of them are rheologically optimal for the functional reconstruction of the vocal fold lamina propria.

Original languageEnglish (US)
Pages (from-to)764-768
Number of pages5
JournalLaryngoscope
Volume120
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

Vocal Cords
Biocompatible Materials
Mucous Membrane
Elastic Modulus
Hyaluronic Acid
Durapatite
Least-Squares Analysis
Tongue
Viscosity
Analysis of Variance
Collagen
Gels
Calcium
Injections
Cymetra
atelocollagen

Keywords

  • Elasticity
  • Injection laryngoplasty
  • Phonosurgery
  • Rheometry
  • Viscosity

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Viscoelastic properties of phonosurgical biomaterials at phonatory frequencies. / Kimura, Miwako; Mau, Ted; Chan, Roger W.

In: Laryngoscope, Vol. 120, No. 4, 04.2010, p. 764-768.

Research output: Contribution to journalArticle

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abstract = "Objectives/Hypothesis: The purpose of this study was to examine the functional biomechanical properties of several injectable biomaterials currently or potentially used for vocal fold augmentation. Study Design: Rheometric investigation of phonosurgical materials in vitro. Methods: Linear viscoelastic shear properties of 3{\%} bovine collagen (atelocollagen), micronized Allo-Derm (Cymetra; LifeCell Corp., Branchburg, NJ), calcium hydroxylapatite (CaHA) (Radiesse; BioForm Medical, San Mateo, CA), and 2.4{\%} cross-linked hyaluronic acid (HA) gel (Juv{\'e}derm; Allergan, Inc., Irvine, CA) were quantified as functions of frequency covering the phonatory range, and compared to those of the human vocal fold cover. Measurements of elastic shear modulus (G′) and dynamic viscosity (η′) were made at up to 250 Hz with a controlled-strain simple-shear rheometer. Linear least-squares regression was conducted to curve-fit log G′ and log η′ versus log frequency, and statistical analysis was performed with one-way analysis of variance. Results: Radiesse and Cymetra were found to be the stiffest and the most viscous materials, followed by Juv{\'e}derm and atelocollagen. There were significant differences in the magnitudes of G′ and η′ among the phonosurgical materials and the normal human vocal fold cover (p < .001), whereas there was no significant difference in the frequency dependence of G′ and η′ among the materials. Post hoc Tukey tests revealed significant differences (p < .05) in pairwise comparisons of the magnitudes of G′ and η′ among all materials and the vocal fold cover. Conclusions: These findings suggested that although these biomaterials may be injected lateral to the lamina propria for the treatment of glottic insufficiency, none of them are rheologically optimal for the functional reconstruction of the vocal fold lamina propria.",
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