Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse

Gina M. Aloisio, Ileana Cuevas, Yuji Nakada, Christopher G. Peña, Diego H. Castrillon

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The precise identity of spermatogonial stem cells—the germline stem cell of the adult testis—remains a controversial topic. Technical limitations have included the lack of specific markers and methods for lineage tracing of Asingle spermatogonia and their subsets. Immunolocalization of proteins in tissue sections has been a standard tool for the in situ identification and visualization of rare cellular subsets. However, these studies are limited by the need for faithful and reliable protein markers to define these cell types, as well as the availability of specific antibodies to these markers. Here we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7+ spermatogonial stem cells and their progeny.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-154
Number of pages16
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Fingerprint

Stem Cells
Spermatogonia
Seminiferous Tubules
Proteins
Monoclonal Antibodies
Antibodies
Adult Germline Stem Cells

Keywords

  • Immunostain
  • Lineage tracing
  • Pax7
  • Spermatogenesis
  • Spermatogonial stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Aloisio, G. M., Cuevas, I., Nakada, Y., Peña, C. G., & Castrillon, D. H. (2017). Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. In Methods in Molecular Biology (Vol. 1463, pp. 139-154). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_11

Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. / Aloisio, Gina M.; Cuevas, Ileana; Nakada, Yuji; Peña, Christopher G.; Castrillon, Diego H.

Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. p. 139-154 (Methods in Molecular Biology; Vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Aloisio, GM, Cuevas, I, Nakada, Y, Peña, CG & Castrillon, DH 2017, Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. in Methods in Molecular Biology. vol. 1463, Methods in Molecular Biology, vol. 1463, Humana Press Inc., pp. 139-154. https://doi.org/10.1007/978-1-4939-4017-2_11
Aloisio GM, Cuevas I, Nakada Y, Peña CG, Castrillon DH. Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. In Methods in Molecular Biology. Vol. 1463. Humana Press Inc. 2017. p. 139-154. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-4017-2_11
Aloisio, Gina M. ; Cuevas, Ileana ; Nakada, Yuji ; Peña, Christopher G. ; Castrillon, Diego H. / Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. pp. 139-154 (Methods in Molecular Biology).
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