Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse

Gina M. Aloisio, Ileana Cuevas, Yuji Nakada, Christopher G. Peña, Diego H. Castrillon

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The precise identity of spermatogonial stem cells—the germline stem cell of the adult testis—remains a controversial topic. Technical limitations have included the lack of specific markers and methods for lineage tracing of Asingle spermatogonia and their subsets. Immunolocalization of proteins in tissue sections has been a standard tool for the in situ identification and visualization of rare cellular subsets. However, these studies are limited by the need for faithful and reliable protein markers to define these cell types, as well as the availability of specific antibodies to these markers. Here we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7+ spermatogonial stem cells and their progeny.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-154
Number of pages16
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Keywords

  • Immunostain
  • Lineage tracing
  • Pax7
  • Spermatogenesis
  • Spermatogonial stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Aloisio, G. M., Cuevas, I., Nakada, Y., Peña, C. G., & Castrillon, D. H. (2017). Visualization and lineage tracing of pax7+ spermatogonial stem cells in the mouse. In Methods in Molecular Biology (Vol. 1463, pp. 139-154). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_11