Visualization of cell-substratum adhesion plaques by antibody exclusion

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We report on an antibody exclusion method for detecting cell adhesion plaques. Substrata coated by plasma fibronectin (pFN) appeared uniformly fluorescent when analyzed by indirect immunofluorescence using a specific anti-pFN antibody. When cells that had spread on the substrata were permeabilized and analyzed by indirect immunofluorescence, areas of non-fluorescence were apparent beneath the cells and in focal streaks along the cell margins. The nonfluorescent focal streaks were coincident with adhesion plaques visualized by interference reflection microscopy.

Original languageEnglish (US)
Pages (from-to)1031-1036
Number of pages6
JournalCell Biology International Reports
Volume4
Issue number11
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Cell Biology

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