TY - JOUR
T1 - Visualizing metal ions in cells
T2 - An overview of analytical techniques, approaches, and probes
AU - Dean, Kevin M.
AU - Qin, Yan
AU - Palmer, Amy E.
N1 - Funding Information:
We would like to thank J. Genevieve Park for careful reading of this manuscript. The authors would like to thank the NIH ( GM084027 to A.E.P.) for financial support. K.M.D. was supported by an NIH Biophysics Training Grant ( T32 GM-065103 ) and NSF IGERT on Computational Optical Sensing Imaging ( 0801680 ).
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/9
Y1 - 2012/9
N2 - Quantifying the amount and defining the location of metal ions in cells and organisms are critical steps in understanding metal homeostasis and how dyshomeostasis causes or is a consequence of disease. A number of recent advances have been made in the development and application of analytical methods to visualize metal ions in biological specimens. Here, we briefly summarize these advances before focusing in more depth on probes for examining transition metals in living cells with high spatial and temporal resolution using fluorescence microscopy. This article is part of a Special Issue entitled: Cell Biology of Metals.
AB - Quantifying the amount and defining the location of metal ions in cells and organisms are critical steps in understanding metal homeostasis and how dyshomeostasis causes or is a consequence of disease. A number of recent advances have been made in the development and application of analytical methods to visualize metal ions in biological specimens. Here, we briefly summarize these advances before focusing in more depth on probes for examining transition metals in living cells with high spatial and temporal resolution using fluorescence microscopy. This article is part of a Special Issue entitled: Cell Biology of Metals.
KW - Fluorescence imaging
KW - Fluorescent sensors
KW - Imaging metal ions in cells
KW - Metal ion homeostasis
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U2 - 10.1016/j.bbamcr.2012.04.001
DO - 10.1016/j.bbamcr.2012.04.001
M3 - Review article
C2 - 22521452
AN - SCOPUS:84864310728
VL - 1823
SP - 1406
EP - 1415
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 9
ER -