Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes

Kevin M. Dean, Yan Qin, Amy E. Palmer

Research output: Contribution to journalReview article

97 Citations (Scopus)

Abstract

Quantifying the amount and defining the location of metal ions in cells and organisms are critical steps in understanding metal homeostasis and how dyshomeostasis causes or is a consequence of disease. A number of recent advances have been made in the development and application of analytical methods to visualize metal ions in biological specimens. Here, we briefly summarize these advances before focusing in more depth on probes for examining transition metals in living cells with high spatial and temporal resolution using fluorescence microscopy. This article is part of a Special Issue entitled: Cell Biology of Metals.

Original languageEnglish (US)
Pages (from-to)1406-1415
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1823
Issue number9
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Fingerprint

Metals
Ions
Fluorescence Microscopy
Cell Biology
Homeostasis

Keywords

  • Fluorescence imaging
  • Fluorescent sensors
  • Imaging metal ions in cells
  • Metal ion homeostasis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Visualizing metal ions in cells : An overview of analytical techniques, approaches, and probes. / Dean, Kevin M.; Qin, Yan; Palmer, Amy E.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1823, No. 9, 01.09.2012, p. 1406-1415.

Research output: Contribution to journalReview article

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