Voltage dependent modification of sodium channel gating with water-soluble carbodiimide

G. N. Mozhayeva, A. P. Naumov, E. D. Nosyreva

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Currents through sodium channels in frog myelinated fibre were measured before and after the treatment of the membrane with watersoluble carbodiimide (WSC). The WSC treatment produces dramatic changes both in activation and inactivation when membrane potential during the treatment is held at levels from -80 to -100 mV. Both gating processes are slowed and their voltage dependence is reduced. The effective charge of activation as measured by limiting logarithmic potential sensitivity is reduced after 10 min of the WSC treatment by the factor of 1.66. The same WSC treatment applied at zero potential induced no change in the effective charge of activation. Other parameters of gating are changed after such treatment but to a lesser degree and in a somewhat different fashion than after the treatment at high negative potential. The results are consistent with the hypothesis that part of a mobile gating charge is presented by carboxyl groups migrating from the external surface to the interior of the channel molecule when the channel opens or inactivates.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalPflügers Archiv European Journal of Physiology
Volume406
Issue number1
DOIs
StatePublished - Jan 1986

Fingerprint

Carbodiimides
Sodium Channels
Water
Chemical activation
Electric potential
Membranes
Anura
Membrane Potentials
Molecules
Fibers

Keywords

  • Carbodiimides
  • Carboxyl groups
  • Mobile charges
  • Nodal membrane
  • Sodium channel
  • Voltage clamp

ASJC Scopus subject areas

  • Physiology

Cite this

Voltage dependent modification of sodium channel gating with water-soluble carbodiimide. / Mozhayeva, G. N.; Naumov, A. P.; Nosyreva, E. D.

In: Pflügers Archiv European Journal of Physiology, Vol. 406, No. 1, 01.1986, p. 31-36.

Research output: Contribution to journalArticle

Mozhayeva, G. N. ; Naumov, A. P. ; Nosyreva, E. D. / Voltage dependent modification of sodium channel gating with water-soluble carbodiimide. In: Pflügers Archiv European Journal of Physiology. 1986 ; Vol. 406, No. 1. pp. 31-36.
@article{2d31417c92a648dc999476e6336dfa0c,
title = "Voltage dependent modification of sodium channel gating with water-soluble carbodiimide",
abstract = "Currents through sodium channels in frog myelinated fibre were measured before and after the treatment of the membrane with watersoluble carbodiimide (WSC). The WSC treatment produces dramatic changes both in activation and inactivation when membrane potential during the treatment is held at levels from -80 to -100 mV. Both gating processes are slowed and their voltage dependence is reduced. The effective charge of activation as measured by limiting logarithmic potential sensitivity is reduced after 10 min of the WSC treatment by the factor of 1.66. The same WSC treatment applied at zero potential induced no change in the effective charge of activation. Other parameters of gating are changed after such treatment but to a lesser degree and in a somewhat different fashion than after the treatment at high negative potential. The results are consistent with the hypothesis that part of a mobile gating charge is presented by carboxyl groups migrating from the external surface to the interior of the channel molecule when the channel opens or inactivates.",
keywords = "Carbodiimides, Carboxyl groups, Mobile charges, Nodal membrane, Sodium channel, Voltage clamp",
author = "Mozhayeva, {G. N.} and Naumov, {A. P.} and Nosyreva, {E. D.}",
year = "1986",
month = "1",
doi = "10.1007/BF00582949",
language = "English (US)",
volume = "406",
pages = "31--36",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Voltage dependent modification of sodium channel gating with water-soluble carbodiimide

AU - Mozhayeva, G. N.

AU - Naumov, A. P.

AU - Nosyreva, E. D.

PY - 1986/1

Y1 - 1986/1

N2 - Currents through sodium channels in frog myelinated fibre were measured before and after the treatment of the membrane with watersoluble carbodiimide (WSC). The WSC treatment produces dramatic changes both in activation and inactivation when membrane potential during the treatment is held at levels from -80 to -100 mV. Both gating processes are slowed and their voltage dependence is reduced. The effective charge of activation as measured by limiting logarithmic potential sensitivity is reduced after 10 min of the WSC treatment by the factor of 1.66. The same WSC treatment applied at zero potential induced no change in the effective charge of activation. Other parameters of gating are changed after such treatment but to a lesser degree and in a somewhat different fashion than after the treatment at high negative potential. The results are consistent with the hypothesis that part of a mobile gating charge is presented by carboxyl groups migrating from the external surface to the interior of the channel molecule when the channel opens or inactivates.

AB - Currents through sodium channels in frog myelinated fibre were measured before and after the treatment of the membrane with watersoluble carbodiimide (WSC). The WSC treatment produces dramatic changes both in activation and inactivation when membrane potential during the treatment is held at levels from -80 to -100 mV. Both gating processes are slowed and their voltage dependence is reduced. The effective charge of activation as measured by limiting logarithmic potential sensitivity is reduced after 10 min of the WSC treatment by the factor of 1.66. The same WSC treatment applied at zero potential induced no change in the effective charge of activation. Other parameters of gating are changed after such treatment but to a lesser degree and in a somewhat different fashion than after the treatment at high negative potential. The results are consistent with the hypothesis that part of a mobile gating charge is presented by carboxyl groups migrating from the external surface to the interior of the channel molecule when the channel opens or inactivates.

KW - Carbodiimides

KW - Carboxyl groups

KW - Mobile charges

KW - Nodal membrane

KW - Sodium channel

KW - Voltage clamp

UR - http://www.scopus.com/inward/record.url?scp=0022658206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022658206&partnerID=8YFLogxK

U2 - 10.1007/BF00582949

DO - 10.1007/BF00582949

M3 - Article

C2 - 2419829

AN - SCOPUS:0022658206

VL - 406

SP - 31

EP - 36

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 1

ER -