TY - JOUR
T1 - Whole-Brain Mapping of Direct Inputs to Midbrain Dopamine Neurons
AU - Watabe-Uchida, Mitsuko
AU - Zhu, Lisa
AU - Ogawa, Sachie K.
AU - Vamanrao, Archana
AU - Uchida, Naoshige
N1 - Funding Information:
We are grateful to Dr. Edward Callaway for providing us with the rabies virus and other reagents and his advice. We thank Dr. John A.T. Young for pCMMP-TVA950. We thank Dr. Catherine Dulac for her support, providing us with various reagents, and her comments on the manuscript. We thank Dr. Joshua Sanes for comments on the manuscript, Drs. Linh Vong and Bradford Lowell for Vgat-ires-Cre mouse, Dr. Edward Boyden for AAV8-CAG-ArchT-GFP, and Dr. Shenqin Yao for pCDNA-mCherry and her technical advice. We thank Neir Eshel, Jeremiah Cohen, and other members of the Uchida lab for critical comments on the manuscript and discussions. This work was supported by a Howard Hughes Medical Institute Collaborative Innovation Award, a Smith Family New Investigator Award, the Alfred Sloan Foundation, and the Milton Fund (N.U.).
PY - 2012/6/7
Y1 - 2012/6/7
N2 - Recent studies indicate that dopamine neurons in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) convey distinct signals. To explore this difference, we comprehensively identified each area@s monosynaptic inputs using the rabies virus. We show that dopamine neurons in both areas integrate inputs from a more diverse collection of areas than previously thought, including autonomic, motor, and somatosensory areas. SNc and VTA dopamine neurons receive contrasting excitatory inputs: the former from the somatosensory/motor cortex and subthalamic nucleus, which may explain their short-latency responses to salient events; and the latter from the lateral hypothalamus, which may explain their involvement in value coding. We demonstrate that neurons in the striatum that project directly to dopamine neurons form patches in both the dorsal and ventral striatum, whereas those projecting to GABAergic neurons are distributed in the matrix compartment. Neuron-type-specific connectivity lays a foundation for studying how dopamine neurons compute outputs.
AB - Recent studies indicate that dopamine neurons in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) convey distinct signals. To explore this difference, we comprehensively identified each area@s monosynaptic inputs using the rabies virus. We show that dopamine neurons in both areas integrate inputs from a more diverse collection of areas than previously thought, including autonomic, motor, and somatosensory areas. SNc and VTA dopamine neurons receive contrasting excitatory inputs: the former from the somatosensory/motor cortex and subthalamic nucleus, which may explain their short-latency responses to salient events; and the latter from the lateral hypothalamus, which may explain their involvement in value coding. We demonstrate that neurons in the striatum that project directly to dopamine neurons form patches in both the dorsal and ventral striatum, whereas those projecting to GABAergic neurons are distributed in the matrix compartment. Neuron-type-specific connectivity lays a foundation for studying how dopamine neurons compute outputs.
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U2 - 10.1016/j.neuron.2012.03.017
DO - 10.1016/j.neuron.2012.03.017
M3 - Article
C2 - 22681690
AN - SCOPUS:84861936797
SN - 0896-6273
VL - 74
SP - 858
EP - 873
JO - Neuron
JF - Neuron
IS - 5
ER -