Whole transcriptome RNA-seq analysis: Tumorigenesis and metastasis of melanoma

Hua Zhao, Yongjun Li, Shaobin Wang, Yadong Yang, Junyun Wang, Xiuyan Ruan, Yaran Yang, Kan Cai, Bing Zhang, Peng Cui, Jiangwei Yan, Yongliang Zhao, Edward K. Wakeland, Quanzhen Li, Songnian Hu, Xiangdong Fang

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Melanoma is the most malignant cutaneous cancer and causes over 9000 deaths annually. Because fatality rates from malignant melanoma (MM) increase dramatically upon metastasis, we investigated tumorigenesis and metastasis of MM in transcriptome analyses of three distinct cell lines that correspond with the stages of MM pathogenesis: the normal stage (HEMn-LP), the onset of MM (A375), and the metastasis stage (A2058). Using next-generation sequencing (NGS) technology, we detected asymmetrical expression of genes among the three cell lines, notably on chromosomes 9, 11, 12, and 14, suggesting their involvement in tumorigenesis and metastasis of MM. These genes were clustered into 41 categories based on their expression patterns, and their biological functions were analyzed using Ingenuity Pathway Analysis. In the top cancer-associated category, HIF1A, IL8, TERT, ONECUT1, and FOXA1 directly interacted with either transcription factors or cytokines that are known to be involved in the tumorigenesis or metastasis of other malignant tumors. The present data suggest that cytokine regulatory pathways in macrophages predominate over other pathways during the pathogenesis of MM. This study provides new targets for the downstream mechanistic studies of the tumorigenesis and metastasis of MM and demonstrates a new strategy for studies of the progression of other malignant cancers.

Original languageEnglish (US)
Pages (from-to)234-243
Number of pages10
JournalGene
Volume548
Issue number2
DOIs
StatePublished - Sep 15 2014

Keywords

  • Melanoma
  • Metastasis
  • Next-generation sequencing
  • Transcriptome
  • Tumorigenesis

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Zhao, H., Li, Y., Wang, S., Yang, Y., Wang, J., Ruan, X., Yang, Y., Cai, K., Zhang, B., Cui, P., Yan, J., Zhao, Y., Wakeland, E. K., Li, Q., Hu, S., & Fang, X. (2014). Whole transcriptome RNA-seq analysis: Tumorigenesis and metastasis of melanoma. Gene, 548(2), 234-243. https://doi.org/10.1016/j.gene.2014.07.038