Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis

Wilbur A. Franklin, Adi F. Gazdar, Jerry Haney, Ignacia I. Wistuba, Francisco G. La Rosa, Tim Kennedy, Donald M. Ritchey, York E. Miller

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

Individuals with one aerodigestive tract malignancy have a high incidence of second primary aerodigestive tumors. The mechanism for this field effect has not been determined. We studied an individual with widespread dysplastic changes in the respiratory epithelium but no overt carcinoma. The entire tracheobronchial tree obtained at autopsy was embedded in paraffin, and bronchial epithelial cells were isolated by microdissection. DNA extracted from the microdissected cells was analyzed for point mutations in the p53 tumor suppressor gene. A single, identical point mutation consisting of a G:C to T:A transversion in codon 245 was identified in bronchial epithelium from 7 of 10 sites in both lungs. Epithelium at sites containing the p53 mutation was morphologically abnormal, exhibiting squamous metaplasia and mild to moderate atypia. No invasive tumor was found in the tracheobronchial tree or any other location. Cells from peripheral blood, kidney, liver, and lymph node exhibited no abnormality in the p53 gene. The widespread presence of a single somatic p53 point mutation in the bronchi of a smoker suggests that a single progenitor bronchial epithelial clone may expand to populate broad areas of the bronchial mucosa-a novel mechanism for field carcinogenesis in the respiratory epithelium that may be of importance in assessing individuals for risk of a second primary tumor as well as in devising effective strategies for chemoprevention of lung cancer.

Original languageEnglish (US)
Pages (from-to)2133-2137
Number of pages5
JournalJournal of Clinical Investigation
Volume100
Issue number8
StatePublished - Oct 15 1997

Fingerprint

Respiratory Mucosa
Carcinogenesis
Point Mutation
Mutation
Neoplasms
Epithelium
Microdissection
p53 Genes
Chemoprevention
Metaplasia
Bronchi
Tumor Suppressor Genes
Codon
Paraffin
Autopsy
Lung Neoplasms
Blood Cells
Mucous Membrane
Clone Cells
Lymph Nodes

Keywords

  • Field cancerization
  • Lung neoplasms
  • Precancerous conditions
  • Smoking
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Franklin, W. A., Gazdar, A. F., Haney, J., Wistuba, I. I., La Rosa, F. G., Kennedy, T., ... Miller, Y. E. (1997). Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis. Journal of Clinical Investigation, 100(8), 2133-2137.

Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis. / Franklin, Wilbur A.; Gazdar, Adi F.; Haney, Jerry; Wistuba, Ignacia I.; La Rosa, Francisco G.; Kennedy, Tim; Ritchey, Donald M.; Miller, York E.

In: Journal of Clinical Investigation, Vol. 100, No. 8, 15.10.1997, p. 2133-2137.

Research output: Contribution to journalArticle

Franklin, WA, Gazdar, AF, Haney, J, Wistuba, II, La Rosa, FG, Kennedy, T, Ritchey, DM & Miller, YE 1997, 'Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis', Journal of Clinical Investigation, vol. 100, no. 8, pp. 2133-2137.
Franklin WA, Gazdar AF, Haney J, Wistuba II, La Rosa FG, Kennedy T et al. Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis. Journal of Clinical Investigation. 1997 Oct 15;100(8):2133-2137.
Franklin, Wilbur A. ; Gazdar, Adi F. ; Haney, Jerry ; Wistuba, Ignacia I. ; La Rosa, Francisco G. ; Kennedy, Tim ; Ritchey, Donald M. ; Miller, York E. / Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis. In: Journal of Clinical Investigation. 1997 ; Vol. 100, No. 8. pp. 2133-2137.
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