TY - JOUR
T1 - Wingless signaling induces nautilus expression in the ventral mesoderm of the Drosophila embryo
AU - Ranganayakulu, Gogineni
AU - Schulz, Robert A.
AU - Olson, Eric N.
N1 - Funding Information:
We are grateful to Roel Nusse for the wg cDNA and antibody, Manfred Frasch for the P[ZKrGAL] line, and Bruce Paterson for the anti-Nau antibody. We thank Helmut Kramer for extending the use of his laboratory facilities. This work was supported by grants from NIH and The Muscular Dystrophy Association to E. Olson, from The Muscular Dystrophy Association to R. A. Schulz, and from the Human Frontiers Science Program to E. Olson, R. Harvey, and R. A. Schulz.
PY - 1996/5/25
Y1 - 1996/5/25
N2 - The segregation of founder cells from the somatic mesoderm is a prerequisite for the formation of body wall muscles in the Drosophila embryo. The myogenic basic helix-loop-helix protein, Nautilus (Nau), is expressed in a subset of these founder cells in medial and lateral positions in the somatic mesoderm. Mutations in the wingless (wg) gene, which encodes a secreted growth factor, lead to the complete loss of Nau-expressing medial muscle precursor cell clusters, but not lateral clusters. Using the GAL4/UAS system, we demonstrate that the wg-derived signal can originate from either ectoderm or mesoderm to influence nau expression. By using a temperature-sensitive wg allele, we also show that wg function is required during and after gastrulation for the formation of Nau-expressing medial muscle precursor cell clusters. Our results, combined with recent studies from chick, suggest a conserved role for Wg signaling pathways during muscle development.
AB - The segregation of founder cells from the somatic mesoderm is a prerequisite for the formation of body wall muscles in the Drosophila embryo. The myogenic basic helix-loop-helix protein, Nautilus (Nau), is expressed in a subset of these founder cells in medial and lateral positions in the somatic mesoderm. Mutations in the wingless (wg) gene, which encodes a secreted growth factor, lead to the complete loss of Nau-expressing medial muscle precursor cell clusters, but not lateral clusters. Using the GAL4/UAS system, we demonstrate that the wg-derived signal can originate from either ectoderm or mesoderm to influence nau expression. By using a temperature-sensitive wg allele, we also show that wg function is required during and after gastrulation for the formation of Nau-expressing medial muscle precursor cell clusters. Our results, combined with recent studies from chick, suggest a conserved role for Wg signaling pathways during muscle development.
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U2 - 10.1006/dbio.1996.9987
DO - 10.1006/dbio.1996.9987
M3 - Article
C2 - 8654890
AN - SCOPUS:0029992508
SN - 0012-1606
VL - 176
SP - 143
EP - 148
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -