Wnt signaling in the murine diastema

Thantrira Porntaveetus, Atsushi Ohazama, Hong Y. Choi, Joachim Herz, Paul T. Sharpe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The correct number and shape of teeth are critical factors for an aesthetic and functional dentition. Understanding the molecular mechanisms regulating tooth number and shape are therefore important in orthodontics. Mice have only one incisor and three molars in each jaw quadrant that are divided by a tooth-less region, the diastema. Although mice lost teeth in the diastema during evolution, the remnants of the evolutionary lost teeth are observed as transient epithelial buds in the wild-type diastema during early stages of development. Shh and Fgf signaling pathways that are essential for tooth development have been shown to be repressed in the diastema. It remains unclear however how Wnt signaling, that is also required for tooth development, is regulated in the diastema. In this study we found that in the embryonic diastema, Wnt5a expression was observed in mesenchyme, whereas Wnt4 and Wnt10b were expressed in epithelium. The expression of Wnt6 and Wnt11 was found in both tissues. The Wnt co-receptor, Lrp6, was weakly expressed in the diastema overlapping with weak Lrp4 expression, a co-receptor that inhibits Wnt signaling . Secreted Wnt inihibitors Dkk1, Dkk2, and Dkk3 were also expressed in the diastema. Lrp4 mutant mice develop supernumerary teeth in the diastema that is accompanied by upregulation of Wnt signaling and Lrp6 expression. Wnt signaling is thus usually attenuated in the diastema by these secreted and membrane bound Wnt inhibitors.

Original languageEnglish (US)
Pages (from-to)518-524
Number of pages7
JournalEuropean Journal of Orthodontics
Volume34
Issue number4
DOIs
StatePublished - Aug 2012

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Diastema
Tooth
Wnt Receptors
Supernumerary Tooth
Dentition
Mesoderm
Incisor
Orthodontics
Jaw
Esthetics
Up-Regulation
Epithelium

ASJC Scopus subject areas

  • Orthodontics

Cite this

Porntaveetus, T., Ohazama, A., Choi, H. Y., Herz, J., & Sharpe, P. T. (2012). Wnt signaling in the murine diastema. European Journal of Orthodontics, 34(4), 518-524. https://doi.org/10.1093/ejo/cjr049

Wnt signaling in the murine diastema. / Porntaveetus, Thantrira; Ohazama, Atsushi; Choi, Hong Y.; Herz, Joachim; Sharpe, Paul T.

In: European Journal of Orthodontics, Vol. 34, No. 4, 08.2012, p. 518-524.

Research output: Contribution to journalArticle

Porntaveetus, T, Ohazama, A, Choi, HY, Herz, J & Sharpe, PT 2012, 'Wnt signaling in the murine diastema', European Journal of Orthodontics, vol. 34, no. 4, pp. 518-524. https://doi.org/10.1093/ejo/cjr049
Porntaveetus T, Ohazama A, Choi HY, Herz J, Sharpe PT. Wnt signaling in the murine diastema. European Journal of Orthodontics. 2012 Aug;34(4):518-524. https://doi.org/10.1093/ejo/cjr049
Porntaveetus, Thantrira ; Ohazama, Atsushi ; Choi, Hong Y. ; Herz, Joachim ; Sharpe, Paul T. / Wnt signaling in the murine diastema. In: European Journal of Orthodontics. 2012 ; Vol. 34, No. 4. pp. 518-524.
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