TY - JOUR
T1 - Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9
AU - Wysocki, Annette B.
AU - Staiano-Coico, Lisa
AU - Grinnell, Frederick
N1 - Funding Information:
These studies were supported by NIH grallis NR06263 (AW), GM26145, GM42461 (LS-C) , atld GM21681 alld GM31321 (FG). Elise Tidwell, R.N., provided expert assistance collectillg chronic wOlmd fluid samples jll Dallas.
PY - 1993/7
Y1 - 1993/7
N2 - The purpose of the present research was to determine if metalloproteinase levels were elevated in human chronic wound fluid. Samples of blood and wound fluid from acute (mastectomy) and chronic (leg ulcer) wounds were collected, and metalloproteinase profiles of the samples were determined by gelatin zymography. Compared to serum, acute wound fluid (mastectomy fluid) contained markedly increased levels (five-to tenfold) of several metalloproteinases including 72-kDa and 94-kDa gelatinases (MMP-2 and MMP-9). In chronic wound fluid, not only were these enzyme levels increased another five-to tenfold over mastectomy fluid, but also both activated enzyme and proenzyme species appeared to be present. Our results suggest that non-healing ulcers develop an environment containing high levels of activated metalloproteinases, which may result in chronic tissue turnover and failed wound closure.
AB - The purpose of the present research was to determine if metalloproteinase levels were elevated in human chronic wound fluid. Samples of blood and wound fluid from acute (mastectomy) and chronic (leg ulcer) wounds were collected, and metalloproteinase profiles of the samples were determined by gelatin zymography. Compared to serum, acute wound fluid (mastectomy fluid) contained markedly increased levels (five-to tenfold) of several metalloproteinases including 72-kDa and 94-kDa gelatinases (MMP-2 and MMP-9). In chronic wound fluid, not only were these enzyme levels increased another five-to tenfold over mastectomy fluid, but also both activated enzyme and proenzyme species appeared to be present. Our results suggest that non-healing ulcers develop an environment containing high levels of activated metalloproteinases, which may result in chronic tissue turnover and failed wound closure.
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U2 - 10.1111/1523-1747.ep12359590
DO - 10.1111/1523-1747.ep12359590
M3 - Article
C2 - 8392530
AN - SCOPUS:0027202705
SN - 0022-202X
VL - 101
SP - 64
EP - 68
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 1
ER -