@article{5593d96d1e0f4fc39305bea391f3f29a,
title = "WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans",
abstract = "During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A β-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit- 1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.",
author = "Rocheleau, {C. E.} and J. Yasuda and {Tae Ho Shin}, {Ho Shin} and R. Lin and H. Sawa and H. Okano and Priess, {J. R.} and Davis, {R. J.} and Mello, {C. C.}",
note = "Funding Information: We thank Ralf Schnabel for providing the lit-1 mutant strains; Hiroko Kouike for excellent technical support; and members of our laboratories for helpful discussions. Some strains were obtained from the C. elegans Genetic Stock Center, which is funded by a grant from the NIH National Center for Research Support. Thanks to the entire Worm Genome Consortium for providing the clones and sequences that made this work possible. Support for C. C. M. was provided in part by a PEW scholarship, a University of Massachusetts DERC pilot and feasibility grant, and an NIH grant. T. S. is a Schering-Plough fellow of the Life Sciences Research Foundation. J. Y., R. J. D., and J. R. P. were supported by the HHMI and by grants from the NIH. Further support for J. Y. was provided by the Foundation for Promotion of Cancer Research. R. L. was supported in part by a Basil O'Connor Grant from the March of Dimes Foundation. ",
year = "1999",
doi = "10.1016/S0092-8674(00)80784-9",
language = "English (US)",
volume = "97",
pages = "717--726",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}