Recent years have brought not only an avalanche of new macromolecular structures, but also significant advances in the protein structure determination methodology only now making its way into structure-based drug discovery. In this chapter, we review recent methodology developments in X-ray diffraction experiments that led to fast and very accurate elucidation of three-dimensional structures of macromolecules. We will discuss the role of data collection as the last experiment performed in the crystal structure determination process. A statistical analysis of diffraction experiments that are reported in the Protein Data Bank (PDB) is also presented.
|Original language||English (US)|
|Number of pages||18|
|Journal||Advances in Protein Chemistry and Structural Biology|
|Publication status||Published - 2009|
ASJC Scopus subject areas
- Structural Biology