Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains

A. Patt, A. H. harken, L. K. Burton, T. C. Rodell, D. Piermattei, W. J. Schorr, N. B. Parker, E. M. Berger, I. R. Horesh, L. S. Terada, S. L. Linas, J. C. Cheronis, J. E. Repine

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Abstract

The contribution of toxic O2 metabolites to cerebral ischemia reperfusion injury has not been determined. We found that gerbils subjected to temporary unilateral carotid artery occlusion (ischemia) consistently developed neurologic deficits during ischemia with severities that correlated with increasing degrees of brain edema and brain H2O2 levels after reperfusion. In contrast, gerbils treated just before reperfusion (after ischemia) with dimethylthiourea (DMTU), but not urea, had decreased brain edema and brain H2O2 levels. In addition, gerbils fed a tungsten-rich diet for 4, 5, or 6 wk developed progressive decreases in brain xanthine oxidase (XO) and brain XO + xanthine dehydrogenase (XD) activities, brain edema, and brain H2O2 levels after temporary unilateral carotid artery occlusion and reperfusion. In contrast to tungsten-treated gerbils, allopurinol-treated gerbils did not have statistically significant decreases in brain XO or XO + XD levels, and reduced brain edema and brain H2O2 levels occurred only in gerbils developing mild but not severe neurologic deficits during ischemia. Finally, gerbils treated with DMTU or tungsten all survived, while > 60% of gerbils treated with urea, allopurinol, or saline die by 48 h after temporary unilateral carotid artery occlusion and reperfusion. Our findings indicate that H2O2 from XO contributes to reperfusion-induced edema in brains subjected to temporary ischemia.

Original languageEnglish (US)
Pages (from-to)1556-1562
Number of pages7
JournalJournal of Clinical Investigation
Volume81
Issue number5
StatePublished - 1988

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Gerbillinae
Xanthine Oxidase
Hydrogen Peroxide
Reperfusion
Edema
Ischemia
Brain Edema
Brain
Tungsten
Carotid Arteries
Xanthine Dehydrogenase
Allopurinol
Neurologic Manifestations
Urea
Poisons
Reperfusion Injury
Brain Ischemia
Diet

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Patt, A., harken, A. H., Burton, L. K., Rodell, T. C., Piermattei, D., Schorr, W. J., ... Repine, J. E. (1988). Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains. Journal of Clinical Investigation, 81(5), 1556-1562.

Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains. / Patt, A.; harken, A. H.; Burton, L. K.; Rodell, T. C.; Piermattei, D.; Schorr, W. J.; Parker, N. B.; Berger, E. M.; Horesh, I. R.; Terada, L. S.; Linas, S. L.; Cheronis, J. C.; Repine, J. E.

In: Journal of Clinical Investigation, Vol. 81, No. 5, 1988, p. 1556-1562.

Research output: Contribution to journalArticle

Patt, A, harken, AH, Burton, LK, Rodell, TC, Piermattei, D, Schorr, WJ, Parker, NB, Berger, EM, Horesh, IR, Terada, LS, Linas, SL, Cheronis, JC & Repine, JE 1988, 'Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains', Journal of Clinical Investigation, vol. 81, no. 5, pp. 1556-1562.
Patt A, harken AH, Burton LK, Rodell TC, Piermattei D, Schorr WJ et al. Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains. Journal of Clinical Investigation. 1988;81(5):1556-1562.
Patt, A. ; harken, A. H. ; Burton, L. K. ; Rodell, T. C. ; Piermattei, D. ; Schorr, W. J. ; Parker, N. B. ; Berger, E. M. ; Horesh, I. R. ; Terada, L. S. ; Linas, S. L. ; Cheronis, J. C. ; Repine, J. E. / Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains. In: Journal of Clinical Investigation. 1988 ; Vol. 81, No. 5. pp. 1556-1562.
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abstract = "The contribution of toxic O2 metabolites to cerebral ischemia reperfusion injury has not been determined. We found that gerbils subjected to temporary unilateral carotid artery occlusion (ischemia) consistently developed neurologic deficits during ischemia with severities that correlated with increasing degrees of brain edema and brain H2O2 levels after reperfusion. In contrast, gerbils treated just before reperfusion (after ischemia) with dimethylthiourea (DMTU), but not urea, had decreased brain edema and brain H2O2 levels. In addition, gerbils fed a tungsten-rich diet for 4, 5, or 6 wk developed progressive decreases in brain xanthine oxidase (XO) and brain XO + xanthine dehydrogenase (XD) activities, brain edema, and brain H2O2 levels after temporary unilateral carotid artery occlusion and reperfusion. In contrast to tungsten-treated gerbils, allopurinol-treated gerbils did not have statistically significant decreases in brain XO or XO + XD levels, and reduced brain edema and brain H2O2 levels occurred only in gerbils developing mild but not severe neurologic deficits during ischemia. Finally, gerbils treated with DMTU or tungsten all survived, while > 60{\%} of gerbils treated with urea, allopurinol, or saline die by 48 h after temporary unilateral carotid artery occlusion and reperfusion. Our findings indicate that H2O2 from XO contributes to reperfusion-induced edema in brains subjected to temporary ischemia.",
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AU - Patt, A.

AU - harken, A. H.

AU - Burton, L. K.

AU - Rodell, T. C.

AU - Piermattei, D.

AU - Schorr, W. J.

AU - Parker, N. B.

AU - Berger, E. M.

AU - Horesh, I. R.

AU - Terada, L. S.

AU - Linas, S. L.

AU - Cheronis, J. C.

AU - Repine, J. E.

PY - 1988

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N2 - The contribution of toxic O2 metabolites to cerebral ischemia reperfusion injury has not been determined. We found that gerbils subjected to temporary unilateral carotid artery occlusion (ischemia) consistently developed neurologic deficits during ischemia with severities that correlated with increasing degrees of brain edema and brain H2O2 levels after reperfusion. In contrast, gerbils treated just before reperfusion (after ischemia) with dimethylthiourea (DMTU), but not urea, had decreased brain edema and brain H2O2 levels. In addition, gerbils fed a tungsten-rich diet for 4, 5, or 6 wk developed progressive decreases in brain xanthine oxidase (XO) and brain XO + xanthine dehydrogenase (XD) activities, brain edema, and brain H2O2 levels after temporary unilateral carotid artery occlusion and reperfusion. In contrast to tungsten-treated gerbils, allopurinol-treated gerbils did not have statistically significant decreases in brain XO or XO + XD levels, and reduced brain edema and brain H2O2 levels occurred only in gerbils developing mild but not severe neurologic deficits during ischemia. Finally, gerbils treated with DMTU or tungsten all survived, while > 60% of gerbils treated with urea, allopurinol, or saline die by 48 h after temporary unilateral carotid artery occlusion and reperfusion. Our findings indicate that H2O2 from XO contributes to reperfusion-induced edema in brains subjected to temporary ischemia.

AB - The contribution of toxic O2 metabolites to cerebral ischemia reperfusion injury has not been determined. We found that gerbils subjected to temporary unilateral carotid artery occlusion (ischemia) consistently developed neurologic deficits during ischemia with severities that correlated with increasing degrees of brain edema and brain H2O2 levels after reperfusion. In contrast, gerbils treated just before reperfusion (after ischemia) with dimethylthiourea (DMTU), but not urea, had decreased brain edema and brain H2O2 levels. In addition, gerbils fed a tungsten-rich diet for 4, 5, or 6 wk developed progressive decreases in brain xanthine oxidase (XO) and brain XO + xanthine dehydrogenase (XD) activities, brain edema, and brain H2O2 levels after temporary unilateral carotid artery occlusion and reperfusion. In contrast to tungsten-treated gerbils, allopurinol-treated gerbils did not have statistically significant decreases in brain XO or XO + XD levels, and reduced brain edema and brain H2O2 levels occurred only in gerbils developing mild but not severe neurologic deficits during ischemia. Finally, gerbils treated with DMTU or tungsten all survived, while > 60% of gerbils treated with urea, allopurinol, or saline die by 48 h after temporary unilateral carotid artery occlusion and reperfusion. Our findings indicate that H2O2 from XO contributes to reperfusion-induced edema in brains subjected to temporary ischemia.

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