Xanthine oxidase does not mediate the antiproliferative effects of interferon-γ in human umbilical vein endothelial cells

L. S. Terada, P. D. Arnold

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3 Citations (Scopus)

Abstract

Interferon-γ (IFN-γ) has potent antiproliferative effects on the endothelium, although the specific mechanisms responsible for this effect are not clear. We tested the hypothesis that suppression of endothelial cell proliferation by IFN-γ is mediated by an increase in xanthine oxidase- derived O2/-·. Human umbilical vein endothelial cells (HUVEC) were exposed to recombinant human IFN-γ. We found that [3H]thymidine uptake decreased (p < 0.05) with increasing doses of IFN-γ. Treatment of HUVEC with the xanthine oxidase inhibitor allopurinol or the O2/-· scavenger superoxide dismutase had no effect (p > 0.05) on [3H]thymidine uptake of IFN-γ-treated cells. In parallel, IFN-γ decreased (p < 0.05) HUVEC cell counts, while allopurinol again had no effect (p > 0.05) on cell counts of IFN-γ-treated or control HUVEC. In addition, xanthine oxidase activity of HUVEC did not (p > 0.05) increase following treatment with IFN-γ. We conclude that IFN-γ suppresses HUVEC proliferation by a mechanism independent of O2/-· production by xanthine oxidase.

Original languageEnglish (US)
Pages (from-to)419-422
Number of pages4
JournalJournal of Interferon Research
Volume13
Issue number6
StatePublished - 1993

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Xanthine Oxidase
Human Umbilical Vein Endothelial Cells
Interferons
Thymidine
Cell Proliferation
Endothelium
Endothelial Cells
Cell Count

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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title = "Xanthine oxidase does not mediate the antiproliferative effects of interferon-γ in human umbilical vein endothelial cells",
abstract = "Interferon-γ (IFN-γ) has potent antiproliferative effects on the endothelium, although the specific mechanisms responsible for this effect are not clear. We tested the hypothesis that suppression of endothelial cell proliferation by IFN-γ is mediated by an increase in xanthine oxidase- derived O2/-·. Human umbilical vein endothelial cells (HUVEC) were exposed to recombinant human IFN-γ. We found that [3H]thymidine uptake decreased (p < 0.05) with increasing doses of IFN-γ. Treatment of HUVEC with the xanthine oxidase inhibitor allopurinol or the O2/-· scavenger superoxide dismutase had no effect (p > 0.05) on [3H]thymidine uptake of IFN-γ-treated cells. In parallel, IFN-γ decreased (p < 0.05) HUVEC cell counts, while allopurinol again had no effect (p > 0.05) on cell counts of IFN-γ-treated or control HUVEC. In addition, xanthine oxidase activity of HUVEC did not (p > 0.05) increase following treatment with IFN-γ. We conclude that IFN-γ suppresses HUVEC proliferation by a mechanism independent of O2/-· production by xanthine oxidase.",
author = "Terada, {L. S.} and Arnold, {P. D.}",
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TY - JOUR

T1 - Xanthine oxidase does not mediate the antiproliferative effects of interferon-γ in human umbilical vein endothelial cells

AU - Terada, L. S.

AU - Arnold, P. D.

PY - 1993

Y1 - 1993

N2 - Interferon-γ (IFN-γ) has potent antiproliferative effects on the endothelium, although the specific mechanisms responsible for this effect are not clear. We tested the hypothesis that suppression of endothelial cell proliferation by IFN-γ is mediated by an increase in xanthine oxidase- derived O2/-·. Human umbilical vein endothelial cells (HUVEC) were exposed to recombinant human IFN-γ. We found that [3H]thymidine uptake decreased (p < 0.05) with increasing doses of IFN-γ. Treatment of HUVEC with the xanthine oxidase inhibitor allopurinol or the O2/-· scavenger superoxide dismutase had no effect (p > 0.05) on [3H]thymidine uptake of IFN-γ-treated cells. In parallel, IFN-γ decreased (p < 0.05) HUVEC cell counts, while allopurinol again had no effect (p > 0.05) on cell counts of IFN-γ-treated or control HUVEC. In addition, xanthine oxidase activity of HUVEC did not (p > 0.05) increase following treatment with IFN-γ. We conclude that IFN-γ suppresses HUVEC proliferation by a mechanism independent of O2/-· production by xanthine oxidase.

AB - Interferon-γ (IFN-γ) has potent antiproliferative effects on the endothelium, although the specific mechanisms responsible for this effect are not clear. We tested the hypothesis that suppression of endothelial cell proliferation by IFN-γ is mediated by an increase in xanthine oxidase- derived O2/-·. Human umbilical vein endothelial cells (HUVEC) were exposed to recombinant human IFN-γ. We found that [3H]thymidine uptake decreased (p < 0.05) with increasing doses of IFN-γ. Treatment of HUVEC with the xanthine oxidase inhibitor allopurinol or the O2/-· scavenger superoxide dismutase had no effect (p > 0.05) on [3H]thymidine uptake of IFN-γ-treated cells. In parallel, IFN-γ decreased (p < 0.05) HUVEC cell counts, while allopurinol again had no effect (p > 0.05) on cell counts of IFN-γ-treated or control HUVEC. In addition, xanthine oxidase activity of HUVEC did not (p > 0.05) increase following treatment with IFN-γ. We conclude that IFN-γ suppresses HUVEC proliferation by a mechanism independent of O2/-· production by xanthine oxidase.

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