Xanthine oxidase produces hydrogen peroxide which contributes to reperfusion injury of ischemic, isolated, perfused rat hearts

J. M. Brown, L. S. Terada, M. A. Grosso, G. J. Whitmann, S. E. Velasco, A. Patt, A. H. Harken, J. E. Repine

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Abstract

Three lines of investigation indicated that hydrogen peroxide (H2O2) from xanthine oxidase (XO) contributes to cardiac dysfunction during reperfusion after ischemia. First, addition of dimethylthiourea (DMTU), a highly permeant O2 metabolite scavenger (but not urea) simultaneously with reperfusion improved recovery of ventricular function as assessed by ventricular developed pressure (DP), contractility (+dP/dt), and relaxation rate (-dP/dt) in isolated Krebs-Henseleit-perfused rat hearts subjects to global normothermic ischemia. Second, hearts from rats fed tungsten or treated with allopurinol had negligible XO activities (0.5 mU/g wet myocardium compared with > 6.0 mU/g in control hearts) and increased ventricular function after ischemia and reperfusion. Third, myocardial H2O2-dependent inactivation of catalase occurred after reperfusion following ischemia, but not after ischemia without reperfusion or perfusion without ischemia. In contrast, myocardial catalase did not decrease during reperfusion of ischemic hearts treated DMTU, tungsten, or allopurinol.

Original languageEnglish (US)
Pages (from-to)1297-1301
Number of pages5
JournalJournal of Clinical Investigation
Volume81
Issue number4
StatePublished - 1988

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Xanthine Oxidase
Reperfusion Injury
Hydrogen Peroxide
Reperfusion
Ischemia
Allopurinol
Tungsten
Ventricular Function
Catalase
Recovery of Function
Ventricular Pressure
Urea
Myocardium
Perfusion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Brown, J. M., Terada, L. S., Grosso, M. A., Whitmann, G. J., Velasco, S. E., Patt, A., ... Repine, J. E. (1988). Xanthine oxidase produces hydrogen peroxide which contributes to reperfusion injury of ischemic, isolated, perfused rat hearts. Journal of Clinical Investigation, 81(4), 1297-1301.

Xanthine oxidase produces hydrogen peroxide which contributes to reperfusion injury of ischemic, isolated, perfused rat hearts. / Brown, J. M.; Terada, L. S.; Grosso, M. A.; Whitmann, G. J.; Velasco, S. E.; Patt, A.; Harken, A. H.; Repine, J. E.

In: Journal of Clinical Investigation, Vol. 81, No. 4, 1988, p. 1297-1301.

Research output: Contribution to journalArticle

Brown, JM, Terada, LS, Grosso, MA, Whitmann, GJ, Velasco, SE, Patt, A, Harken, AH & Repine, JE 1988, 'Xanthine oxidase produces hydrogen peroxide which contributes to reperfusion injury of ischemic, isolated, perfused rat hearts', Journal of Clinical Investigation, vol. 81, no. 4, pp. 1297-1301.
Brown, J. M. ; Terada, L. S. ; Grosso, M. A. ; Whitmann, G. J. ; Velasco, S. E. ; Patt, A. ; Harken, A. H. ; Repine, J. E. / Xanthine oxidase produces hydrogen peroxide which contributes to reperfusion injury of ischemic, isolated, perfused rat hearts. In: Journal of Clinical Investigation. 1988 ; Vol. 81, No. 4. pp. 1297-1301.
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AU - Brown, J. M.

AU - Terada, L. S.

AU - Grosso, M. A.

AU - Whitmann, G. J.

AU - Velasco, S. E.

AU - Patt, A.

AU - Harken, A. H.

AU - Repine, J. E.

PY - 1988

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N2 - Three lines of investigation indicated that hydrogen peroxide (H2O2) from xanthine oxidase (XO) contributes to cardiac dysfunction during reperfusion after ischemia. First, addition of dimethylthiourea (DMTU), a highly permeant O2 metabolite scavenger (but not urea) simultaneously with reperfusion improved recovery of ventricular function as assessed by ventricular developed pressure (DP), contractility (+dP/dt), and relaxation rate (-dP/dt) in isolated Krebs-Henseleit-perfused rat hearts subjects to global normothermic ischemia. Second, hearts from rats fed tungsten or treated with allopurinol had negligible XO activities (0.5 mU/g wet myocardium compared with > 6.0 mU/g in control hearts) and increased ventricular function after ischemia and reperfusion. Third, myocardial H2O2-dependent inactivation of catalase occurred after reperfusion following ischemia, but not after ischemia without reperfusion or perfusion without ischemia. In contrast, myocardial catalase did not decrease during reperfusion of ischemic hearts treated DMTU, tungsten, or allopurinol.

AB - Three lines of investigation indicated that hydrogen peroxide (H2O2) from xanthine oxidase (XO) contributes to cardiac dysfunction during reperfusion after ischemia. First, addition of dimethylthiourea (DMTU), a highly permeant O2 metabolite scavenger (but not urea) simultaneously with reperfusion improved recovery of ventricular function as assessed by ventricular developed pressure (DP), contractility (+dP/dt), and relaxation rate (-dP/dt) in isolated Krebs-Henseleit-perfused rat hearts subjects to global normothermic ischemia. Second, hearts from rats fed tungsten or treated with allopurinol had negligible XO activities (0.5 mU/g wet myocardium compared with > 6.0 mU/g in control hearts) and increased ventricular function after ischemia and reperfusion. Third, myocardial H2O2-dependent inactivation of catalase occurred after reperfusion following ischemia, but not after ischemia without reperfusion or perfusion without ischemia. In contrast, myocardial catalase did not decrease during reperfusion of ischemic hearts treated DMTU, tungsten, or allopurinol.

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