Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs

H. K. Moores, C. J. Beehler, M. E. Hanley, P. F. Shanley, E. E. Stevens, J. E. Repine, L. S. Terada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Neutrophil accumulation in alveolar spaces is a conspicuous finding in hyperoxia-exposed lungs. We hypothesized that xanthine oxidase (XO)-derived oxidants contribute to retention of neutrophils in hyperoxic lungs. Rats were subjected to normobaric hyperoxia (100% O2) for 48 h, and lungs were assessed for neutrophil sequestration (morphometry and lavage cell counts) and injury (lavage albumin levels and lung weights). In rats exposed to hyperoxia, we found increased (P < 0.05) lung neutrophil retention, lavage albumin levels, and lung weights compared with normoxia-exposed control rats. Suppression of XO activity by pretreatment with allopurinol decreased (P < 0.05) lung neutrophil retention but increased (P < 0.05) lavage albumin concentrations and lung weights in hyperoxic rats. Allopurinol treatment had no effect (P > 0.05) on the numbers of macrophages or lymphocytes recoverable by lung lavage. Depletion of XO activity by an independent method, tungsten feeding, also decreased (P < 0.05) lung lavage neutrophil counts and increased (P < 0.05) lavage albumin concentrations. We conclude that XO may be involved in lung neutrophil retention but not lung injury during exposure to hyperoxia.

Original languageEnglish (US)
Pages (from-to)941-945
Number of pages5
JournalJournal of Applied Physiology
Volume76
Issue number2
StatePublished - 1994

Fingerprint

Xanthine Oxidase
Hyperoxia
Neutrophils
Therapeutic Irrigation
Lung
Wounds and Injuries
Bronchoalveolar Lavage
Albumins
Feeding Methods
Tungsten
Lung Injury
Oxidants
Cell Count
Macrophages
Lymphocytes
Weights and Measures

Keywords

  • allopurinol
  • hyperoxia
  • inflammation
  • tungsten

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Moores, H. K., Beehler, C. J., Hanley, M. E., Shanley, P. F., Stevens, E. E., Repine, J. E., & Terada, L. S. (1994). Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs. Journal of Applied Physiology, 76(2), 941-945.

Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs. / Moores, H. K.; Beehler, C. J.; Hanley, M. E.; Shanley, P. F.; Stevens, E. E.; Repine, J. E.; Terada, L. S.

In: Journal of Applied Physiology, Vol. 76, No. 2, 1994, p. 941-945.

Research output: Contribution to journalArticle

Moores, HK, Beehler, CJ, Hanley, ME, Shanley, PF, Stevens, EE, Repine, JE & Terada, LS 1994, 'Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs', Journal of Applied Physiology, vol. 76, no. 2, pp. 941-945.
Moores HK, Beehler CJ, Hanley ME, Shanley PF, Stevens EE, Repine JE et al. Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs. Journal of Applied Physiology. 1994;76(2):941-945.
Moores, H. K. ; Beehler, C. J. ; Hanley, M. E. ; Shanley, P. F. ; Stevens, E. E. ; Repine, J. E. ; Terada, L. S. / Xanthine oxidase promotes neutrophil sequestration but not injury in hyperoxic lungs. In: Journal of Applied Physiology. 1994 ; Vol. 76, No. 2. pp. 941-945.
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