Xanthomonas type III effector XopD targets SUMO-conjugated proteins in planta

Andrew Hotson, Renee Chosed, Hongjun Shu, Kim Orth, Mary Beth Mudgett

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197 Scopus citations

Abstract

Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provide direct evidence for a functional role for a phytopathogenic bacterial TTSS effector in planta by demonstrating that the Xcv effector XopD encodes an active cysteine protease with plant-specific SUMO substrate specificity. XopD is injected into plant cells by the TTSS during Xcv pathogenesis, translocated to subnuclear foci and hydrolyses SUMO-conjugated proteins in vivo. Our studies suggest that XopD mimics endogenous plant SUMO isopeptidases to interfere with the regulation of host proteins during Xcv infection.

Original languageEnglish (US)
Pages (from-to)377-389
Number of pages13
JournalMolecular Microbiology
Volume50
Issue number2
DOIs
StatePublished - Oct 1 2003

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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