XRCC3 is required for efficient repair of chromosome breaks by homologous recombination

Mark A. Brenneman, Anne E. Weiss, Jac A. Nickoloff, David J. Chen

Research output: Contribution to journalArticle

154 Scopus citations

Abstract

XRCC3 was originally identified as a human gene able to complement the DNA damage sensitivity, chromosomal instability and impaired growth of the mutant hamster cell line irs1SF. More recently, it has been cloned, sequenced and found to bear sequence homology to the highly conserved eukaryotic repair and recombination gene RAD51. The phenotype of irs1SF and the identification of XRCC3 as a member of the RAD51 gene family have suggested a role for XRCC3 in repair of DNA damage by homologous recombination. Homologous recombinational repair (HRR) of a specifically induced chromosomal double-strand break (DSB) was assayed in irs1SF cells with and without transient complementation by human XRCC3. Complementation with XRCC3 increased the frequencies of repair by 34- to 260-fold. The results confirm a role for XRCC3 in HRR of DNA DSB, and the importance of this repair pathway for the maintenance of chromosomal integrity in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalMutation Research - DNA Repair
Volume459
Issue number2
DOIs
StatePublished - Mar 20 2000

Keywords

  • Chromosomal stability
  • Chromosome breaks
  • DNA double-strand breaks
  • DNA repair
  • Homologous recombination
  • XRCC3

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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