Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins: Implications for the structure and function of transcription/ repair factor b

Lee Bardwell, A. Jane Bardwell, W. John Feaver, Jesper Q. Svejstrup, Roger D. Kornberg, Errol C. Friedberg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The RAD3 and SSL2 gene products are essential proteins that are also required for the nucleotide excision repair pathway. We have recently demonstrated that the RAD3 gene product along with the SSL1 and TFB1 gene products are subunits of RNA polymerase II basal transcription factor b. Additionally, the SSL2 gene product physically interacts with purified factor b. Here we combine an in vitro immunoprccipitation assay and an in vivo genetic assay to demonstrate a series of pairwise protein-protein interactions involving these components. RAD3 protein binds directly to both SSL2 protein and SSL1 protein in vitro. SSL1 protein interacts with itself and with RAD3 and TFB1 proteins in living yeast cells. An N-terminal, possibly noncatalytic, domain of SSL2 protein is sufficient for the factor b-SSL2 interaction, and a product of a DNA repair-defective allele of SSL2 is not defective in binding to factor b. We present genetic evidence suggesting that the DNA-repair function of SSL2 protein is not dependent on its essential function.

Original languageEnglish (US)
Pages (from-to)3926-3930
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number9
StatePublished - Apr 26 1994

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Fungal Proteins
Transcription Factors
Proteins
DNA Repair
Genes
RNA Polymerase II
Yeasts
Alleles

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins : Implications for the structure and function of transcription/ repair factor b. / Bardwell, Lee; Bardwell, A. Jane; Feaver, W. John; Svejstrup, Jesper Q.; Kornberg, Roger D.; Friedberg, Errol C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 9, 26.04.1994, p. 3926-3930.

Research output: Contribution to journalArticle

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