Yeast two-hybrid studies

M. J. Smith; K. Pozo; F. A. Stephenson

doi:10.1007/978-0-387-30401-4_19

Yeast two-hybrid studies

M. J. Smith, K. Pozo, F. A. Stephenson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The yeast two-hybrid system is a high throughput method for the study of protein-protein interactions. The use and application of two GAL4 based and the CytoTrap Sos recruitment yeast two-hybrid systems are described. This includes a detailed consideration of appropriate baits together with their generation and characterization for use in the screening of cDNA libraries for the detection of interacting proteins, the characterization of positive clones resulting from cDNA library screens and strategies for the validation of protein-protein interactions using alternative experimental paradigms.

Original language	English (US)
Title of host publication	Handbook of Neurochemistry and Molecular Neurobiology
Subtitle of host publication	Practical Neurochemistry Methods
Publisher	Springer US
Pages	409-421
Number of pages	13
ISBN (Print)	9780387303598
DOIs	https://doi.org/10.1007/978-0-387-30401-4_19
State	Published - 2007

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology

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10.1007/978-0-387-30401-4_19

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Yeast two-hybrid studies

Abstract

ASJC Scopus subject areas

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