Yeast two-hybrid studies

M. J. Smith, K. Pozo, F. A. Stephenson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The yeast two-hybrid system is a high throughput method for the study of protein-protein interactions. The use and application of two GAL4 based and the CytoTrap Sos recruitment yeast two-hybrid systems are described. This includes a detailed consideration of appropriate baits together with their generation and characterization for use in the screening of cDNA libraries for the detection of interacting proteins, the characterization of positive clones resulting from cDNA library screens and strategies for the validation of protein-protein interactions using alternative experimental paradigms.

Original languageEnglish (US)
Title of host publicationHandbook of Neurochemistry and Molecular Neurobiology
Subtitle of host publicationPractical Neurochemistry Methods
PublisherSpringer US
Pages409-421
Number of pages13
ISBN (Print)9780387303598
DOIs
Publication statusPublished - Dec 1 2007

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Smith, M. J., Pozo, K., & Stephenson, F. A. (2007). Yeast two-hybrid studies. In Handbook of Neurochemistry and Molecular Neurobiology: Practical Neurochemistry Methods (pp. 409-421). Springer US. https://doi.org/10.1007/978-0-387-30401-4_19