ZBP-89, a kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter

Juanita L. Merchant, Gaitry R. Iyer, Brigit R. Taylor, John R. Kitchen, Eric R. Mortensen, Ziyuan Wang, Rebecca J. Flintoft, Jeffrey B. Michel, Rhonda Bassel-Duby

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111 Scopus citations

Abstract

We have shown previously that a GC-rich element (GGGGCGGGGTGGGGGG) conferring epidermal growth factor (EGF) responsiveness to the human gastrin promoter binds Sp1 and additional undefined complexes. A rat GH4 cell line expression library, was screened by using a multimer of the gastrin EGF response element, and three overlapping cDNA clones were identified. The full-length rat cDNA encoded an 89-kDa zinc linger protein (ZBP-89) that was 89% identical to a 49-kDa human factor, htβ, that hinds a GTGGG/CACCC element in T-cell receptor promoters. The conservation of amino acids between the zinc fingers indicates that ZBP-89 is a member of the C2H2 zinc finger family subclass typified by the Drosophila Kruppel protein. ZBP-89 is ubiquitously expressed in normal adult tissues. It binds specifically to the gastrin EGF response element and inhibits EGF induction of the gastrin promoter. Collectively, these results demonstrate that ZBP-89 functions as a repressor of basal and inducible expression of the gastrin gene.

Original languageEnglish (US)
Pages (from-to)6644-6653
Number of pages10
JournalMolecular and Cellular Biology
Volume16
Issue number12
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Merchant, J. L., Iyer, G. R., Taylor, B. R., Kitchen, J. R., Mortensen, E. R., Wang, Z., ... Bassel-Duby, R. (1996). ZBP-89, a kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter. Molecular and Cellular Biology, 16(12), 6644-6653.