Zebrafish as a model for the study of solid malignancies

Genevieve C. Kendall, James F. Amatruda

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Zebrafish cancer models have provided critical insight into understanding the link between aberrant developmental pathways and tumorigenesis. The unique strengths of zebrafish as compared to other vertebrate model systems include the combination of fecundity, readily available and efficient transgenesis techniques, transparency that facilitates in vivo cell lineage tracing, and amenability for high-throughput applications. In addition to early embryo readouts, zebrafish can develop tumors at ages ranging from 2 weeks old to adulthood. Tumorigenesis is driven by genetically introducing oncogenes using selected promoter/tissue-specific expression, with either mosaic expression or with the generation of a stable transgenic line. Here, we detail a research pipeline to facilitate the study of human oncogenes in zebrafish systems. The goals of this approach are to identify conserved developmental pathways that may be critical for tumor development and to create platforms for testing novel therapies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-142
Number of pages22
Volume1451
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1451
ISSN (Print)10643745

Keywords

  • Functional genomics
  • Gateway cloning
  • Histopathology
  • Solid malignancies
  • Tol2
  • Transgenesis
  • Zebrafish tumor model

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Zebrafish as a model for the study of solid malignancies'. Together they form a unique fingerprint.

  • Cite this

    Kendall, G. C., & Amatruda, J. F. (2016). Zebrafish as a model for the study of solid malignancies. In Methods in Molecular Biology (Vol. 1451, pp. 121-142). (Methods in Molecular Biology; Vol. 1451). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3771-4_9