Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision

Sandi Jo Estill, Joseph A. Garcia

Research output: Contribution to journalArticle

676 Citations (Scopus)

Abstract

The Cre/loxP system has become an important tool in designing postintegrational switch mechanisms for transgenes in mice. The power and spectrum of application of this system depends on transgenic mouse lines that provide Cre recombinase activity with a defined cell type-, tissue-, or developmental stage-specificity. We have developed a novel mouse line that acts as a Cre reporter. The mice, designated Z/EG (lacZl EGFP), express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to indicate the occurrence of Cre excision from early embryonic to adult lineages. The advantage of the Z/EG line is that Cre-mediated excision can be monitored in live samples and that live cells with Cre-mediated excision can be isolated using a single-step FACS. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system.

Original languageEnglish (US)
Pages (from-to)147-155
Number of pages9
JournalGenesis
Volume28
Issue number3-4
DOIs
StatePublished - 2000

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Lac Operon
Transgenes
Transgenic Mice
Embryonic Development
Research Personnel
enhanced green fluorescent protein
Site-specific recombinase
Cre recombinase

Keywords

  • Conditional gene expression
  • Cre
  • Enhanced green fluorescent protein
  • IoxP
  • LacZ
  • Transgenic mice

ASJC Scopus subject areas

  • Genetics

Cite this

Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. / Estill, Sandi Jo; Garcia, Joseph A.

In: Genesis, Vol. 28, No. 3-4, 2000, p. 147-155.

Research output: Contribution to journalArticle

Estill, Sandi Jo ; Garcia, Joseph A. / Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. In: Genesis. 2000 ; Vol. 28, No. 3-4. pp. 147-155.
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