Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells

Rana K Gupta, Rina J. Mepani, Sandra Kleiner, James C. Lo, Melin J. Khandekar, Paul Cohen, Andrea Frontini, Diti Chatterjee Bhowmick, Li Ye, Saverio Cinti, Bruce M. Spiegelman

Research output: Contribution to journalArticle

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Abstract

Progress has been made in elucidating the cell-surface phenotype of primary adipose progenitors; however, specific functional markers and distinct molecular signatures of fat depot-specific preadipocytes have remained elusive. In this study, we label committed murine adipose progenitors through expression of GFP from the genetic locus for Zfp423, a gene controlling preadipocyte determination. Selection of GFP-expressing fibroblasts from either subcutaneous or visceral adipose-derived stromal vascular cultures isolates stably committed preadipocytes that undergo robust adipogenesis. Immunohistochemistry for Zfp423-driven GFP expression in vivo confirms a perivascular origin of preadipocytes within both white and brown adipose tissues. Interestingly, a small subset of capillary endothelial cells within white and brown fat also express this marker, suggesting a contribution of specialized endothelial cells to the adipose lineage. Zfp423 GFP mice represent a simple tool for the specific localization and isolation of molecularly defined preadipocytes from distinct adipose tissue depots.

Original languageEnglish (US)
Pages (from-to)230-239
Number of pages10
JournalCell Metabolism
Volume15
Issue number2
DOIs
StatePublished - Feb 8 2012

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White Adipose Tissue
Brown Adipose Tissue
Endothelial Cells
Adipogenesis
Genetic Loci
Blood Vessels
Adipose Tissue
Fibroblasts
Immunohistochemistry
Fats
Phenotype
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. / Gupta, Rana K; Mepani, Rina J.; Kleiner, Sandra; Lo, James C.; Khandekar, Melin J.; Cohen, Paul; Frontini, Andrea; Bhowmick, Diti Chatterjee; Ye, Li; Cinti, Saverio; Spiegelman, Bruce M.

In: Cell Metabolism, Vol. 15, No. 2, 08.02.2012, p. 230-239.

Research output: Contribution to journalArticle

Gupta, RK, Mepani, RJ, Kleiner, S, Lo, JC, Khandekar, MJ, Cohen, P, Frontini, A, Bhowmick, DC, Ye, L, Cinti, S & Spiegelman, BM 2012, 'Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells', Cell Metabolism, vol. 15, no. 2, pp. 230-239. https://doi.org/10.1016/j.cmet.2012.01.010
Gupta, Rana K ; Mepani, Rina J. ; Kleiner, Sandra ; Lo, James C. ; Khandekar, Melin J. ; Cohen, Paul ; Frontini, Andrea ; Bhowmick, Diti Chatterjee ; Ye, Li ; Cinti, Saverio ; Spiegelman, Bruce M. / Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. In: Cell Metabolism. 2012 ; Vol. 15, No. 2. pp. 230-239.
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