Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells

Rana K Gupta, Rina J. Mepani, Sandra Kleiner, James C. Lo, Melin J. Khandekar, Paul Cohen, Andrea Frontini, Diti Chatterjee Bhowmick, Li Ye, Saverio Cinti, Bruce M. Spiegelman

Research output: Contribution to journalArticle

239 Scopus citations

Abstract

Progress has been made in elucidating the cell-surface phenotype of primary adipose progenitors; however, specific functional markers and distinct molecular signatures of fat depot-specific preadipocytes have remained elusive. In this study, we label committed murine adipose progenitors through expression of GFP from the genetic locus for Zfp423, a gene controlling preadipocyte determination. Selection of GFP-expressing fibroblasts from either subcutaneous or visceral adipose-derived stromal vascular cultures isolates stably committed preadipocytes that undergo robust adipogenesis. Immunohistochemistry for Zfp423-driven GFP expression in vivo confirms a perivascular origin of preadipocytes within both white and brown adipose tissues. Interestingly, a small subset of capillary endothelial cells within white and brown fat also express this marker, suggesting a contribution of specialized endothelial cells to the adipose lineage. Zfp423 GFP mice represent a simple tool for the specific localization and isolation of molecularly defined preadipocytes from distinct adipose tissue depots.

Original languageEnglish (US)
Pages (from-to)230-239
Number of pages10
JournalCell Metabolism
Volume15
Issue number2
DOIs
StatePublished - Feb 8 2012

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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    Gupta, R. K., Mepani, R. J., Kleiner, S., Lo, J. C., Khandekar, M. J., Cohen, P., Frontini, A., Bhowmick, D. C., Ye, L., Cinti, S., & Spiegelman, B. M. (2012). Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. Cell Metabolism, 15(2), 230-239. https://doi.org/10.1016/j.cmet.2012.01.010