ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet α Cell Heterogeneity

Ebrahim H. Ghazvini Zadeh; Zhi Jiang Huang; Jing Xia; Daliang Li; Howard W. Davidson; Wen hong Li

doi:10.1016/j.celrep.2020.107904

ZIGIR, a Granule-Specific Zn²⁺ Indicator, Reveals Human Islet α Cell Heterogeneity

Ebrahim H. Ghazvini Zadeh, Zhi Jiang Huang, Jing Xia, Daliang Li, Howard W. Davidson, Wen hong Li

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Ghazvini Zadeh et al. develop a fluorescent zinc indictor, ZIGIR, that labels Zn²⁺-rich secretory granules with high specificity and sensitivity. ZIGIR tracks trafficking and exocytosis of native granules, enables sorting of islet α cells and β cells with high purity, and reveals human α cell heterogeneity and high Zn²⁺ activity in the human somatostatin granule.

Original language	English (US)
Article number	107904
Journal	Cell Reports
Volume	32
Issue number	2
DOIs	https://doi.org/10.1016/j.celrep.2020.107904
State	Published - Jul 14 2020

Keywords

FACS islet cell
TIRF imaging
ZIGIR
Zinc sensor
ZnT8
alpha cell heterogeneity
beta cell
delta cell
dense core granule
zinc imaging

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/j.celrep.2020.107904

Cite this

@article{58a342a48c7a4492812dc8e172a19389,

title = "ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet α Cell Heterogeneity",

abstract = "Ghazvini Zadeh et al. develop a fluorescent zinc indictor, ZIGIR, that labels Zn2+-rich secretory granules with high specificity and sensitivity. ZIGIR tracks trafficking and exocytosis of native granules, enables sorting of islet α cells and β cells with high purity, and reveals human α cell heterogeneity and high Zn2+ activity in the human somatostatin granule.",

keywords = "FACS islet cell, TIRF imaging, ZIGIR, Zinc sensor, ZnT8, alpha cell heterogeneity, beta cell, delta cell, dense core granule, zinc imaging",

author = "{Ghazvini Zadeh}, {Ebrahim H.} and Huang, {Zhi Jiang} and Jing Xia and Daliang Li and Davidson, {Howard W.} and Li, {Wen hong}",

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AU - Ghazvini Zadeh, Ebrahim H.

AU - Huang, Zhi Jiang

AU - Xia, Jing

AU - Li, Daliang

AU - Davidson, Howard W.

AU - Li, Wen hong

PY - 2020/7/14

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KW - TIRF imaging

KW - ZIGIR

KW - Zinc sensor

KW - ZnT8

KW - alpha cell heterogeneity

KW - beta cell

KW - delta cell

KW - dense core granule

KW - zinc imaging

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