ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet α Cell Heterogeneity

Ebrahim H. Ghazvini Zadeh, Zhi Jiang Huang, Jing Xia, Daliang Li, Howard W. Davidson, Wen hong Li

Research output: Contribution to journalArticle

Abstract

Ghazvini Zadeh et al. develop a fluorescent zinc indictor, ZIGIR, that labels Zn2+-rich secretory granules with high specificity and sensitivity. ZIGIR tracks trafficking and exocytosis of native granules, enables sorting of islet α cells and β cells with high purity, and reveals human α cell heterogeneity and high Zn2+ activity in the human somatostatin granule.

Original languageEnglish (US)
Article number107904
JournalCell Reports
Volume32
Issue number2
DOIs
StatePublished - Jul 14 2020

Keywords

  • alpha cell heterogeneity
  • beta cell
  • delta cell
  • dense core granule
  • FACS islet cell
  • TIRF imaging
  • ZIGIR
  • zinc imaging
  • Zinc sensor
  • ZnT8

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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