Microbiology - Alto Lab

Research Output 1999 2019

2019

A NIK–SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-κB

Liu, Z., Mar, K. B., Hanners, N. W., Perelman, S. S., Kanchwala, M., Xing, C., Schoggins, J. W. & Alto, N. M., Jan 1 2019, In : Nature.

Research output: Contribution to journalLetter

Transcription Factors
Inflammation
Organogenesis
Homeobox Genes
Immune System Diseases
1 Citation (Scopus)

Screening Mycobacterium tuberculosis secreted proteins identifies Mpt64 as a eukaryotic membrane-binding bacterial effector

Stamm, C. E., Pasko, B. L., Chaisavaneeyakorn, S., Franco, L. H., Nair, V. R., Weigele, B. A., Alto, N. M. & Shiloh, M. U., May 1 2019, In : mSphere. 4, 3, e00354-19.

Research output: Contribution to journalArticle

Open Access
Mycobacterium tuberculosis
Membranes
Proteins
Macrophages
Unfolded Protein Response
2018
5 Citations (Scopus)

Cooperative immune suppression by Escherichia coli and Shigella effector proteins

de Jong, M. F. & Alto, N. M., Apr 1 2018, In : Infection and Immunity. 86, 4, e00560-17.

Research output: Contribution to journalShort survey

Shigella
Escherichia coli
Infection
Enterohemorrhagic Escherichia coli
Immune Evasion
2017
10 Citations (Scopus)

A systematic exploration of the interactions between bacterial effector proteins and host cell membranes

Weigele, B. A., Orchard, R. C., Jimenez, A., Cox, G. W. & Alto, N. M., Dec 1 2017, In : Nature Communications. 8, 1, 532.

Research output: Contribution to journalArticle

effectors
Bacterial Proteins
Cell membranes
Cell Membrane
proteins
2016
5 Citations (Scopus)

Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection

Perelman, S. S., Abrams, M. E., Eitson, J. L., Chen, D., Jimenez, A., Mettlen, M., Schoggins, J. W. & Alto, N. M., Dec 21 2016, In : PLoS Pathogens. 12, 12, e1006102.

Research output: Contribution to journalArticle

Listeriosis
Listeria monocytogenes
Interferons
Genes
Interferon Type I
10 Citations (Scopus)

How Bacteria Subvert Animal Cell Structure and Function

Jimenez, A., Chen, D. & Alto, N. M., Oct 6 2016, In : Annual Review of Cell and Developmental Biology. 32, p. 373-397 25 p.

Research output: Contribution to journalArticle

Bacterial Toxins
Animal Structures
Host-Pathogen Interactions
Bacterial Proteins
Cell Shape
31 Citations (Scopus)

Shigella flexneri suppresses NF-κB activation by inhibiting linear ubiquitin chain ligation

De Jong, M. F., Liu, Z., Chen, D. & Alto, N. M., May 27 2016, In : Nature Microbiology. 1, 7, 16084.

Research output: Contribution to journalArticle

Shigella flexneri
Ubiquitin
Ligation
Ubiquitination
Ubiquitin-Protein Ligases
2015
24 Citations (Scopus)

Myristoylome profiling reveals a concerted mechanism of ARF GTPase deacylation by the bacterial protease IpaJ

Burnaevskiy, N., Peng, T., Reddick, L. E., Hang, H. C. & Alto, N. M., Apr 2 2015, In : Molecular Cell. 58, 1, p. 110-122 13 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
Peptide Hydrolases
Proteins
Shigella flexneri
Essential Fatty Acids
104 Citations (Scopus)

STING activation by translocation from the ER is associated with infection and autoinflammatory disease

Dobbs, N., Burnaevskiy, N., Chen, D., Gonugunta, V. K., Alto, N. M. & Yan, N., Aug 12 2015, In : Cell Host and Microbe. 18, 2, p. 157-168 12 p.

Research output: Contribution to journalArticle

Inborn Genetic Diseases
Shigella
Medical Genetics
Autoimmunity
Signal Transduction
2014
69 Citations (Scopus)

Bacteria fighting back: How pathogens target and subvert the host innate immune system

Reddick, L. E. & Alto, N. M., Apr 24 2014, In : Molecular Cell. 54, 2, p. 321-328 8 p.

Research output: Contribution to journalArticle

Pattern Recognition Receptors
Protein Transport
Innate Immunity
Immune System
Signal Transduction
17 Citations (Scopus)

Enteropathogenic E. coli effectors EspG1/G2 disrupt microtubules, contribute to tight junction perturbation and inhibit restoration

Glotfelty, L. G., Zahs, A., Hodges, K., Shan, K., Alto, N. M. & Hecht, G. A., Dec 1 2014, In : Cellular Microbiology. 16, 12, p. 1767-1783 17 p.

Research output: Contribution to journalArticle

Enteropathogenic Escherichia coli
Tight Junctions
Microtubules
Escherichia coli Infections
ADP-Ribosylation Factors
19 Citations (Scopus)

Selective protection of an ARF1-GTP signaling axis by a bacterial scaffold induces bidirectional trafficking arrest

Selyunin, A. S., Reddick, L. E., Weigele, B. A. & Alto, N. M., 2014, In : Cell Reports. 6, 5, p. 878-891 14 p.

Research output: Contribution to journalArticle

Escherichia coli Proteins
Guanosine Triphosphate
Scaffolds
Endoplasmic Reticulum
Escherichia coli
2 Citations (Scopus)

Thinking outside the Osp(G) - Kinase activation by E2-ubiquitin

De Jong, M. F. & Alto, N. M., Mar 3 2014, In : EMBO Journal. 33, 5, p. 403-404 2 p.

Research output: Contribution to journalArticle

Cyclic GMP-Dependent Protein Kinases
Shigella
Ubiquitin
Phosphotransferases
Chemical activation
2013
73 Citations (Scopus)

Proteolytic elimination of N-myristoyl modifications by the Shigella virulence factor IpaJ

Burnaevskiy, N., Fox, T. G., Plymire, D. A., Ertelt, J. M., Weigele, B. A., Selyunin, A. S., Way, S. S., Patrie, S. M. & Alto, N. M., Apr 4 2013, In : Nature. 496, 7443, p. 106-109 4 p.

Research output: Contribution to journalArticle

Shigella
Virulence Factors
Plasmids
Antigens
Proteins
2012
Electron microscopy
Optical microscopy
Electron Microscopy
Light
Molecules
17 Citations (Scopus)

Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification

Gawthorne, J. A., Reddick, L. E., Akpunarlieva, S. N., Beckham, K. S. H., Christie, J. M., Alto, N. M., Gabrielsen, M. & Roe, A. J., Dec 27 2012, In : PLoS One. 7, 12, e52962.

Research output: Contribution to journalArticle

flavoproteins
Flavoproteins
Purification
protein synthesis
Oxygen
20 Citations (Scopus)

Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit

Orchard, R. C., Kittisopikul, M., Altschuler, S. J., Wu, L. F., Süel, G. M. & Alto, N. M., Feb 17 2012, In : Cell. 148, 4, p. 803-815 13 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
Actins
Networks (circuits)
Theoretical Models
Cells
26 Citations (Scopus)

Mimicking GEFs: A common theme for bacterial pathogens

Orchard, R. C. & Alto, N. M., Jan 2012, In : Cellular Microbiology. 14, 1, p. 10-18 9 p.

Research output: Contribution to journalArticle

Guanine Nucleotide Exchange Factors
GTP Phosphohydrolases
Cell Physiological Phenomena
rho GTP-Binding Proteins
Virulence Factors
53 Citations (Scopus)

Subversion of cell signaling by pathogens

Alto, N. M. & Orth, K., Sep 2012, In : Cold Spring Harbor perspectives in biology. 4, 9

Research output: Contribution to journalArticle

Cell signaling
Cell Shape
Pathogens
GTP-Binding Proteins
Innate Immunity

Subversion of cell signaling by pathogens.

Alto, N. M. & Orth, K., Sep 2012, In : Cold Spring Harbor perspectives in biology. 4, 9

Research output: Contribution to journalArticle

Cell signaling
Cell Shape
Pathogens
GTP-Binding Proteins
Innate Immunity
2011
9 Citations (Scopus)

Activation of PAK by a bacterial type III effector EspG reveals alternative mechanisms of GTPase pathway regulation

Selyunin, A. S. & Alto, N. M., Jul 2011, In : Small GTPases. 2, 4, p. 217-221 5 p.

Research output: Contribution to journalArticle

rho GTP-Binding Proteins
GTP Phosphohydrolases
Virulence Factors
Chemical activation
Enterohemorrhagic Escherichia coli
82 Citations (Scopus)

The assembly of a GTPase-kinase signalling complex by a bacterial catalytic scaffold

Selyunin, A. S., Sutton, S. E., Weigele, B. A., Reddick, L. E., Orchard, R. C., Bresson, S. M., Tomchick, D. R. & Alto, N. M., Jan 6 2011, In : Nature. 469, 7328, p. 107-113 7 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
ADP-Ribosylation Factors
p21-Activated Kinases
Phosphotransferases
Enterohemorrhagic Escherichia coli
2010
23 Citations (Scopus)

E. coli secreted protein F promotes EPEC invasion of intestinal epithelial cells via an SNX9-dependent mechanism

Weflen, A. W., Alto, N. M., Viswanathan, V. K. & Hecht, G., Jul 2010, In : Cellular Microbiology. 12, 7, p. 919-929 11 p.

Research output: Contribution to journalArticle

Sorting Nexins
Enteropathogenic Escherichia coli
Escherichia coli Proteins
Epithelial Cells
Dynamins
94 Citations (Scopus)

Rab32 modulates apoptosis onset and Mitochondria-associated Membrane (MAM) properties

Bui, M., Gilady, S. Y., Fitzsimmons, R. E. B., Benson, M. D., Lynes, E. M., Gesson, K., Alto, N. M., Strack, S., Scott, J. D. & Simmen, T., Oct 8 2010, In : Journal of Biological Chemistry. 285, 41, p. 31590-31602 13 p.

Research output: Contribution to journalArticle

Mitochondria
Endoplasmic Reticulum
Apoptosis
Membranes
Calnexin
2 Citations (Scopus)

Salmonella taking charge

Weigele, B. A. & Alto, N. M., Jun 17 2010, In : Cell Host and Microbe. 7, 6, p. 421-422 2 p.

Research output: Contribution to journalArticle

Bacterial Proteins
Salmonella
Membranes
2009
96 Citations (Scopus)

Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics

Huang, Z., Sutton, S. E., Wallenfang, A. J., Orchard, R. C., Wu, X., Feng, Y., Chai, J. & Alto, N. M., Aug 2009, In : Nature Structural and Molecular Biology. 16, 8, p. 853-860 8 p.

Research output: Contribution to journalArticle

Guanine Nucleotide Exchange Factors
GTP Phosphohydrolases
Protein Isoforms
rho GTP-Binding Proteins
Shigella
18 Citations (Scopus)

Tight junctions and enteropathogenic E. coli

Weflen, A. W., Alto, N. M. & Hecht, G. A., May 2009, Annals of the New York Academy of Sciences. Vol. 1165. p. 169-174 6 p. (Annals of the New York Academy of Sciences; vol. 1165).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sorting Nexins
Enteropathogenic Escherichia coli
Tight Junctions
Escherichia coli Proteins
Escherichia coli
2008
5 Citations (Scopus)

Analysis of Rho-GTPase Mimicry by a Family of Bacterial Type III Effector Proteins

Alto, N. M. & Dixon, J. E., 2008, In : Methods in Enzymology. 439, p. 131-143 13 p.

Research output: Contribution to journalArticle

rho GTP-Binding Proteins
Actins
Pathogens
Proteins
Actin Cytoskeleton
14 Citations (Scopus)

Mimicking small G-proteins: An emerging theme from the bacterial virulence arsenal

Alto, N. M., Mar 2008, In : Cellular Microbiology. 10, 3, p. 566-575 10 p.

Research output: Contribution to journalArticle

Arsenals
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Virulence
Communicable Diseases
112 Citations (Scopus)

Structure and Function of Salmonella SifA Indicate that Its Interactions with SKIP, SseJ, and RhoA Family GTPases Induce Endosomal Tubulation

Ohlson, M. B., Huang, Z., Alto, N. M., Blanc, M. P., Dixon, J. E., Chai, J. & Miller, S. I., Nov 13 2008, In : Cell Host and Microbe. 4, 5, p. 434-446 13 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
Salmonella
Rho Guanine Nucleotide Exchange Factors
Kinesin
Phagosomes
3 Citations (Scopus)

Subversion of Myosin Function by E. coli

Mattoo, S., Alto, N. M. & Dixon, J. E., Jan 15 2008, In : Developmental Cell. 14, 1, p. 8-10 3 p.

Research output: Contribution to journalArticle

Myosins
Escherichia coli
Actins
Bacterial Translocation
Enteropathogenic Escherichia coli
47 Citations (Scopus)

The enteropathogenic Escherichia coli effector protein EspF decreases sodium hydrogen exchanger 3 activity

Hodges, K., Alto, N. M., Ramaswamy, K., Dudeja, P. K. & Hecht, G., Aug 2008, In : Cellular Microbiology. 10, 8, p. 1735-1745 11 p.

Research output: Contribution to journalArticle

Enteropathogenic Escherichia coli
Escherichia coli Proteins
Fibroblasts
Escherichia coli
Sorting Nexins
2007

Shigella Puts the Brakes on the Host Cell Cycle

Alto, N. M., Sep 13 2007, In : Cell Host and Microbe. 2, 3, p. 141-142 2 p.

Research output: Contribution to journalArticle

Shigella
Eukaryotic Cells
Bacterial Infections
Cell Cycle
Epithelium
93 Citations (Scopus)

The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways

Alto, N. M., Weflen, A. W., Rardin, M. J., Yarar, D., Lazar, C. S., Tonikian, R., Koller, A., Taylor, S. S., Boone, C., Sidhu, S. S., Schmid, S. L., Hecht, G. A. & Dixon, J. E., Sep 24 2007, In : Journal of Cell Biology. 178, 7, p. 1265-1278 14 p.

Research output: Contribution to journalArticle

Neuronal Wiskott-Aldrich Syndrome Protein
Sorting Nexins
Enteropathogenic Escherichia coli
Membranes
Bacterial Toxins
2006
189 Citations (Scopus)

Identification of a bacterial type III effector family with G protein mimicry functions

Alto, N. M., Shao, F., Lazar, C. S., Brost, R. L., Chua, G., Mattoo, S., McMahon, S. A., Ghosh, P., Hughes, T. R., Boone, C. & Dixon, J. E., Jan 13 2006, In : Cell. 124, 1, p. 133-145 13 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
Pathogens
GTP-Binding Proteins
Enteropathogenic Escherichia coli
ras Proteins
2005
86 Citations (Scopus)

Functions of the Yersinia effector proteins in inhibiting host immune responses

Navarro, L., Alto, N. M. & Dixon, J. E., Feb 2005, In : Current Opinion in Microbiology. 8, 1, p. 21-27 7 p.

Research output: Contribution to journalArticle

Yersinia
Microbial Genes
Proteins
Actin Cytoskeleton
Phagocytosis
2004
7 Citations (Scopus)

The role of A-Kinase anchoring proteins in cAMP-mediated signal transduction pathways.

Alto, N. M. & Scott, J. D., 2004, In : Cell Biochemistry and Biophysics. 40, 3 Suppl, p. 201-208 8 p.

Research output: Contribution to journalArticle

Signal transduction
Protein Kinases
Signal Transduction
Phosphotransferases
Cyclic AMP-Dependent Protein Kinases
2003
136 Citations (Scopus)

Bioinformatic design of A-kinase anchoring protein-in silico: A potent and selective peptide antagonist of type II protein kinase A anchoring

Alto, N. M., Soderling, S. H., Hoshi, N., Langeberg, L. K., Fayos, R., Jennings, P. A. & Scott, J. D., Apr 15 2003, In : Proceedings of the National Academy of Sciences of the United States of America. 100, 8, p. 4445-4450 6 p.

Research output: Contribution to journalArticle

Cyclic AMP-Dependent Protein Kinase Type II
Computational Biology
Computer Simulation
Protein Kinases
Peptides
26 Citations (Scopus)

Fluid flow regulates E-selectin protein levels in human endothelial cells by inhibiting translation

Kraiss, L. W., Alto, N. M., Dixon, D. A., McIntyre, T. M., Weyrich, A. S. & Zimmerman, G. A., Jan 1 2003, In : Journal of Vascular Surgery. 37, 1, p. 161-168 8 p.

Research output: Contribution to journalArticle

E-Selectin
Endothelial Cells
Proteins
Tumor Necrosis Factor-alpha
Human Umbilical Vein Endothelial Cells
2002
39 Citations (Scopus)

Intracellular targeting of protein kinases and phosphatases

Alto, N., Carlisle Michel, J. J., Dodge, K. L., Langeberg, L. K. & Scott, J. D., Dec 1 2002, In : Diabetes. 51, SUPPL. 3

Research output: Contribution to journalArticle

Phosphoprotein Phosphatases
Protein Kinases
Second Messenger Systems
Cyclic AMP-Dependent Protein Kinases
Phosphoric Monoester Hydrolases
161 Citations (Scopus)

Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics

Alto, N. M., Soderling, J. & Scott, J. D., Aug 19 2002, In : Journal of Cell Biology. 158, 4, p. 659-668 10 p.

Research output: Contribution to journalArticle

Mitochondrial Dynamics
Protein Kinases
Mitochondria
Microtubule-Organizing Center
Mitochondrial Proteins
2001
23 Citations (Scopus)

Flow-induced DNA synthesis requires signaling to a translational control pathway

Kraiss, L. W., Ennis, T. M. & Alto, N. M., 2001, In : Journal of Surgical Research. 97, 1, p. 20-26 7 p.

Research output: Contribution to journalArticle

Sirolimus
Human Umbilical Vein Endothelial Cells
DNA
Mitogens
Thymidine
2000
23 Citations (Scopus)

A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis

Pultz, M. A., Zimmerman, K. K., Alto, N. M., Kaeberlein, M., Lange, S. K., Pitt, J. N., Reeves, N. L. & Zehrung, D. L., Mar 2000, In : Genetics. 154, 3, p. 1213-1229 17 p.

Research output: Contribution to journalArticle

Wasps
Drosophila
Mutation
Phenotype
Hymenoptera
63 Citations (Scopus)

Fluid flow activates a regulator of translation, p70/p85 S6 kinase, in human endothelial cells

Kraiss, L. W., Weyrich, A. S., Alto, N. M., Dixon, D. A., Ennis, T. M., Modur, V., McIntyre, T. M., Prescott, S. M. & Zimmerman, G. A., May 2000, In : American Journal of Physiology - Heart and Circulatory Physiology. 278, 5 47-5

Research output: Contribution to journalArticle

70-kDa Ribosomal Protein S6 Kinases
Sirolimus
Endothelial Cells
Ribosomal Protein S6 Kinases
Protein Biosynthesis
170 Citations (Scopus)

Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold

Westphal, R. S., Soderling, S. H., Alto, N. M., Langeberg, L. K. & Scott, J. D., Sep 1 2000, In : EMBO Journal. 19, 17, p. 4589-4600 12 p.

Research output: Contribution to journalArticle

Wiskott-Aldrich Syndrome Protein
Wiskott-Aldrich Syndrome Protein Family
Scaffolds
Protein Kinases
Cicatrix
1999
35 Citations (Scopus)

Extensive zygotic control of the anteroposterior axis in the wasp Nasonia vitripennis

Pultz, M. A., Pitt, J. N. & Alto, N. M., Feb 1999, In : Development. 126, 4, p. 701-710 10 p.

Research output: Contribution to journalArticle

Wasps
Drosophila
Mothers
Embryonic Structures
Genes
406 Citations (Scopus)

Regulation of NMDA receptors by an associated phosphatase-kinase signaling complex

Westphal, R. S., Tavalin, S. J., Lin, J. W., Alto, N. M., Fraser, I. D. C., Langeberg, L. K., Sheng, M. & Scott, J. D., Jul 2 1999, In : Science. 285, 5424, p. 93-96 4 p.

Research output: Contribution to journalArticle

N-Methyl-D-Aspartate Receptors
Phosphoric Monoester Hydrolases
Phosphotransferases
Protein Phosphatase 1
Holoenzymes