Pharmacology - Chook Lab

Research Output 1990 2019

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2019

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Padavannil, A., Sarkar, P., Kim, S. J., Cagatay, T., Jiou, J., Brautigam, C. A., Tomchick, D. R., Sali, A., D'Arcy, S. & Chook, Y. M., Mar 11 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Histone Chaperones
Karyopherins
Nucleosomes
Histones
DNA
1 Citation (Scopus)
Open Access
Nuclear Export Signals
Cell Nucleus Active Transport
Binding Sites
Peptides
Proteins
2018
12 Citations (Scopus)

Active nuclear import and passive nuclear export are the primary determinants of TDP-43 localization

Pinarbasi, E. S., Caǧatay, T., Fung, H. Y. J., Li, Y. C., Chook, Y. M. & Thomas, P. J., Dec 1 2018, In : Scientific Reports. 8, 1, 7083.

Research output: Contribution to journalArticle

Nuclear Export Signals
Cell Nucleus Active Transport
Nuclear Fusion
Karyopherins
RNA-Binding Proteins
1 Citation (Scopus)

Correlation of CRM1-NES affinity with nuclear export activity

Fu, S. C., Fung, H. Y. J., Caǧatay, T., Baumhardt, J. & Chook, Y. M., Aug 15 2018, In : Molecular Biology of the Cell. 29, 17, p. 2037-2044 8 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Cell Nucleus Active Transport
Peptides
Mutant Proteins
Protein Kinase Inhibitors

Günter Blobel 1936–2018

Chook, Y. M., Fontoura, B. M. A. & Rout, M. P., Apr 2 2018, (Accepted/In press) In : Nature Structural and Molecular Biology. p. 1-2 2 p.

Research output: Contribution to journalArticle

38 Citations (Scopus)

Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites

Yoshizawa, T., Ali, R., Jiou, J., Fung, H. Y. J., Burke, K. A., Kim, S. J., Lin, Y., Peeples, W. B., Saltzberg, D., Soniat, M., Baumhardt, J. M., Oldenbourg, R., Sali, A., Fawzi, N. L., Rosen, M. K. & Chook, Y. M., Jan 1 2018, (Accepted/In press) In : Cell.

Research output: Contribution to journalArticle

Karyopherins
Cell Nucleus Active Transport
Cytoplasmic and Nuclear Receptors
Phase separation
Sarcoma
56 Citations (Scopus)

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

Guo, L., Kim, H. J., Wang, H., Monaghan, J., Freyermuth, F., Sung, J. C., O'Donovan, K., Fare, C. M., Diaz, Z., Singh, N., Zhang, Z. C., Coughlin, M., Sweeny, E. A., DeSantis, M. E., Jackrel, M. E., Rodell, C. B., Burdick, J. A., King, O. D., Gitler, A. D., Lagier-Tourenne, C. & 4 others, Pandey, U. B., Chook, Y. M., Taylor, J. P. & Shorter, J., Apr 19 2018, In : Cell. 173, 3, p. 677-692.e20

Research output: Contribution to journalArticle

Karyopherins
RNA-Binding Proteins
Cell Nucleus Active Transport
Prions
Phase Transition
1 Citation (Scopus)

Structural–functional interactions of NS1-BP protein with the splicing and mRNA export machineries for viral and host gene expression

Zhang, K., Shang, G., Padavannil, A., Wang, J., Sakthivel, R., Chen, X., Kim, M., Thompson, M. G., García-Sastre, A., Lynch, K. W., Chen, Z., Chook, Y. M. & Fontoura, B. M., Dec 26 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 52, p. E12218-E12227

Research output: Contribution to journalArticle

Protein Splicing
Viral Genes
Gene Expression
Messenger RNA
Cell Nucleus Active Transport
2017
15 Citations (Scopus)

Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals

Fung, H. Y. J., Fu, S. C. & Chook, Y. M., Mar 10 2017, In : eLife. 6, e23961.

Research output: Contribution to journalArticle

Nuclear Export Signals
Cell Nucleus Active Transport
Cytoplasmic and Nuclear Receptors
Conformations
Peptides
2016
11 Citations (Scopus)

Importin 8 mediates m7G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E

Volpon, L., Culjkovic-Kraljacic, B., Osborne, M. J., Ramteke, A., Sun, Q., Niesman, A., Chook, Y. M. & Borden, K. L. B., May 10 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 19, p. 5263-5268 6 p.

Research output: Contribution to journalArticle

Eukaryotic Initiation Factor-4E
Karyopherins
Eukaryotic Initiation Factors
Cell Nucleus Active Transport
Guanosine
9 Citations (Scopus)

Karyopherin-β2 Recognition of a PY-NLS Variant that Lacks the Proline-Tyrosine Motif

Soniat, M. & Chook, Y. M., Oct 4 2016, In : Structure. 24, 10, p. 1802-1809 8 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Proline
Tyrosine
Epitopes
33 Citations (Scopus)

Next-generation XPO1 inhibitor shows improved efficacy and in vivo tolerability in hematological malignancies

Hing, Z. A., Fung, H. Y. J., Ranganathan, P., Mitchell, S., El-Gamal, D., Woyach, J. A., Williams, K., Goettl, V. M., Smith, J., Yu, X., Meng, X., Sun, Q., Cagatay, T., Lehman, A. M., Lucas, D. M., Baloglu, E., Shacham, S., Kauffman, M. G., Byrd, J. C., Chook, Y. M. & 2 others, Garzon, R. & Lapalombella, R., Jun 21 2016, (Accepted/In press) In : Leukemia.

Research output: Contribution to journalArticle

Hematologic Neoplasms
Cell Nucleus Active Transport
B-Cell Chronic Lymphocytic Leukemia
Acute Myeloid Leukemia
Tumor Suppressor Proteins
13 Citations (Scopus)

Recognition elements in the histone H3 and H4 tails for seven different importins

Soniat, M., Caǧatay, T. & Chook, Y. M., Sep 30 2016, In : Journal of Biological Chemistry. 291, 40, p. 21171-21183 13 p.

Research output: Contribution to journalArticle

Karyopherins
Histones
Lysine
Histone Chaperones
Acetylation
2015
32 Citations (Scopus)

Nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination

Haines, J. D., Herbin, O., De La Hera, B., Vidaurre, O. G., Moy, G. A., Sun, Q., Fung, H. Y. J., Albrecht, S., Alexandropoulos, K., McCauley, D., Chook, Y. M., Kuhlmann, T., Kidd, G. J., Shacham, S. & Casaccia, P., Apr 28 2015, In : Nature Neuroscience. 18, 4, p. 511-520 10 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Demyelinating Diseases
Gene Knockdown Techniques
Neurons
Kainic Acid
50 Citations (Scopus)

Nuclear localization signals for four distinct Karyopherin-β nuclear import systems

Soniat, M. & Chook, Y. M., Jun 15 2015, In : Biochemical Journal. 468, 3, p. 353-362 10 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Cell Nucleus Active Transport
Proline
Tyrosine
27 Citations (Scopus)

Structural determinants of nuclear export signal orientation in binding to exportin CRM1

Fung, H. Y. J., Fu, S. C., Brautigam, C. A. & Chook, Y. M., Sep 8 2015, In : eLife. 4, September 2015, e10034.

Research output: Contribution to journalArticle

Nuclear Export Signals
Karyopherins
Chromosomes
Maintenance
Protein Sorting Signals
2014
58 Citations (Scopus)

Atomic basis of CRM1-cargo recognition, release and inhibition

Fung, H. Y. J. & Chook, Y. M., 2014, In : Seminars in Cancer Biology. 27, p. 52-61 10 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Cytoplasm
Short Interspersed Nucleotide Elements
Nuclear Export Signals
Neoplasms
41 Citations (Scopus)

LocNES: A computational tool for locating classical NESs in CRM1 cargo proteins

Xu, D., Marquis, K., Pei, J., Fu, S. C., Caʇatay, T., Grishin, N. V. & Chook, Y. M., Nov 5 2014, In : Bioinformatics. 31, 9, p. 1357-1365 9 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Peptides
Proteins
Protein
Nuclear Proteins
15 Citations (Scopus)

Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

Bernis, C., Swift-Taylor, B., Nord, M., Carmona, S., Chook, Y. M. & Forbes, D. J., Apr 1 2014, In : Molecular Biology of the Cell. 25, 7, p. 992-1009 18 p.

Research output: Contribution to journalArticle

Karyopherins
Chromatin
Nuclear Pore
Kinetochores
Nuclear Pore Complex Proteins
2013
101 Citations (Scopus)

Antileukemic activity of nuclear export inhibitors that spare normal hematopoietic cells

Etchin, J., Sun, Q., Kentsis, A., Farmer, A., Zhang, Z. C., Sanda, T., Mansour, M. R., Barcelo, C., McCauley, D., Kauffman, M., Shacham, S., Christie, A. L., Kung, A. L., Rodig, S. J., Chook, Y. M. & Look, A. T., Jan 2013, In : Leukemia. 27, 1, p. 66-74 9 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Nuclear Export Signals
Acute Myeloid Leukemia
Myeloid Cells
Digital Libraries
9 Citations (Scopus)

Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

Soniat, M., Sampathkumar, P., Collett, G., Gizzi, A. S., Banu, R. N., Bhosle, R. C., Chamala, S., Chowdhury, S., Fiser, A., Glenn, A. S., Hammonds, J., Hillerich, B., Khafizov, K., Love, J. D., Matikainen, B., Seidel, R. D., Toro, R., Rajesh Kumar, P., Bonanno, J. B., Chook, Y. M. & 1 others, Almo, S. C., Jun 2013, In : Journal of Structural and Functional Genomics. 14, 2, p. 31-35 5 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Yeast
Saccharomyces cerevisiae
Crystal structure
81 Citations (Scopus)

Nuclear export inhibition through covalent conjugation and hydrolysis of Leptomycin B by CRM1

Sun, Q., Carrasco, Y. P., Hu, Y., Guo, X., Mirzaei, H., MacMillan, J. & Chook, Y. M., Jan 22 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 4, p. 1303-1308 6 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Hydrolysis
Maintenance
Biological Products
Nuclear Export Signals
2012
83 Citations (Scopus)

NESdb: A database of NES-containing CRM1 cargoes

Xu, D., Grishin, N. V. & Chook, Y. M., Sep 15 2012, In : Molecular Biology of the Cell. 23, 18, p. 3673-3676 4 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Databases
Nonprofit Organizations
Karyopherins
Cell Nucleus Active Transport
159 Citations (Scopus)

Selective inhibitors of nuclear export show that CRM1/XPO1 is a target in chronic lymphocytic leukemia

Lapalombella, R., Sun, Q., Williams, K., Tangeman, L., Jha, S., Zhong, Y., Goettl, V., Mahoney, E., Berglund, C., Gupta, S., Farmer, A., Mani, R., Johnson, A. J., Lucas, D., Mo, X., Daelemans, D., Sandanayaka, V., Shechter, S., McCauley, D., Shacham, S. & 3 others, Kauffman, M., Chook, Y. M. & Byrd, J. C., Nov 29 2012, In : Blood. 120, 23, p. 4621-4634 14 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
B-Cell Chronic Lymphocytic Leukemia
Toxicity
Tumor Suppressor Proteins
Cysteine
60 Citations (Scopus)

Sequence and structural analyses of nuclear export signals in the NESdb database

Xu, D., Farmer, A., Collett, G., Grishin, N. V. & Chook, Y. M., Sep 15 2012, In : Molecular Biology of the Cell. 23, 18, p. 3677-3693 17 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Sequence Analysis
Databases
Amino Acids
Position-Specific Scoring Matrices
64 Citations (Scopus)
Nuclear Localization Signals
Amyotrophic Lateral Sclerosis
Proline
Sarcoma
Tyrosine
2011
16 Citations (Scopus)

Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1

Zhang, Z. C., Satterly, N., Fontoura, B. M. A. & Chook, Y. M., Dec 1 2011, In : Molecular Biology of the Cell. 22, 23, p. 4657-4668 12 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Messenger RNA
Cell Nucleus Active Transport
Eukaryota
21 Citations (Scopus)

Importin-α protein binding to a nuclear localization signal of carbohydrate response element-binding protein (ChREBP)

Ge, Q., Nakagawa, T., Max Wynn, R., Chook, Y. M., Miller, B. C. & Uyeda, K., Aug 12 2011, In : Journal of Biological Chemistry. 286, 32, p. 28119-28127 9 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Response Elements
Protein Binding
Carrier Proteins
211 Citations (Scopus)

Nuclear import by karyopherin-βs: Recognition and inhibition

Chook, Y. M. & Süel, K. E., Sep 2011, In : Biochimica et Biophysica Acta - Molecular Cell Research. 1813, 9, p. 1593-1606 14 p.

Research output: Contribution to journalArticle

Karyopherins
Cell Nucleus Active Transport
Cytoplasm
Nuclear Localization Signals
Nuclear Proteins
2010
136 Citations (Scopus)

Recognition of nuclear targeting signals by Karyopherin-β proteins

Xu, D., Farmer, A. & Chook, Y. M., Dec 2010, In : Current Opinion in Structural Biology. 20, 6, p. 782-790 9 p.

Research output: Contribution to journalArticle

Karyopherins
Cell Nucleus Active Transport
Proteins
Nuclear Export Signals
Nuclear Localization Signals
2009

Erratum: Structural basis for leucine-rich nuclear export signal recognition by CRM1 (Nature (2009) 458 (1136-1141))

Dong, X., Biswas, A., Süel, K. E., Jackson, L. K., Martinez, R., Gu, H. & Chook, Y. M., Sep 24 2009, In : Nature. 461, 7263, p. 550 1 p.

Research output: Contribution to journalArticle

20 Citations (Scopus)

Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus

Süel, K. E. & Chook, Y. M., Jun 5 2009, In : Journal of Biological Chemistry. 284, 23, p. 15416-15424 9 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Transcription Factors
Karyopherins
DNA
Bioinformatics
64 Citations (Scopus)

Structural basis for assembly and disassembly of the CRM1 nuclear export complex

Dong, X., Biswas, A. & Chook, Y. M., May 2009, In : Nature Structural and Molecular Biology. 16, 5, p. 558-560 3 p.

Research output: Contribution to journalArticle

Nuclear Pore
Cell Nucleus Active Transport
Guanosine Triphosphate
Cell Nucleus
Carrier Proteins
195 Citations (Scopus)

Structural basis for leucine-rich nuclear export signal recognition by CRM1

Dong, X., Biswas, A., Süel, K. E., Jackson, L. K., Martinez, R., Gu, H. & Chook, Y. M., Apr 30 2009, In : Nature. 458, 7242, p. 1136-1141 6 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Leucine
Cell Nucleus Active Transport
Epitopes
Nucleotides
2008
53 Citations (Scopus)

Modular organization and combinatorial energetics of proline-tyrosine nuclear localization signals

Süel, K. E., Gu, H. & Chook, Y. M., Jun 2008, In : PLoS Biology. 6, 6, p. 1253-1267 15 p., e137.

Research output: Contribution to journalArticle

Nuclear Localization Signals
nuclear localization signals
Proline
Tyrosine
tyrosine
2007
25 Citations (Scopus)

Conformational Heterogeneity of Karyopherinβ2 Is Segmental

Cansizoglu, A. E. & Chook, Y. M., Nov 13 2007, In : Structure. 15, 11, p. 1431-1441 11 p.

Research output: Contribution to journalArticle

Karyopherins
Binding Sites
Nuclear Pore
RNA-Binding Proteins
Cytoplasm
100 Citations (Scopus)

Structure-based design of a pathway-specific nuclear import inhibitor

Cansizoglu, A. E., Lee, B. J., Zhang, Z. C., Fontoura, B. M. A. & Chook, Y. M., May 2007, In : Nature Structural and Molecular Biology. 14, 5, p. 452-454 3 p.

Research output: Contribution to journalArticle

Nuclear Localization Signals
Cell Nucleus Active Transport
Karyopherins
Ligands
2006
23 Citations (Scopus)

Atomic resolution structures in nuclear transport

Süel, K. E., Cansizoglu, A. E. & Chook, Y. M., Aug 2006, In : Methods. 39, 4, p. 342-355 14 p.

Research output: Contribution to journalArticle

Cell Nucleus Active Transport
Carrier Proteins
Nuclear Proteins
Mutagenesis
Recombinant Proteins
322 Citations (Scopus)

Rules for Nuclear Localization Sequence Recognition by Karyopherinβ2

Lee, B. J., Cansizoglu, A. E., Süel, K. E., Louis, T. H., Zhang, Z. & Chook, Y. M., Aug 4 2006, In : Cell. 126, 3, p. 543-558 16 p.

Research output: Contribution to journalArticle

Karyopherins
GTP Phosphohydrolases
Substrates
Nuclear Export Signals
Nuclear Localization Signals
63 Citations (Scopus)

The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions

Faria, A. M. C., Levay, A., Wang, Y., Kamphorst, A. O., Rosa, M. L. P., Nussenzveig, D. R., Balkan, W., Chook, Y. M., Levy, D. E. & Fontoura, B. M. A., Mar 2006, In : Immunity. 24, 3, p. 295-304 10 p.

Research output: Contribution to journalArticle

Interferons
Proteins
nuclear pore complex protein 96
T-Lymphocytes
Cell Nucleus Active Transport
2004
97 Citations (Scopus)

AMP-activated protein kinase-regulated phosphorylation and acetylation of importin α1: Involvement in the nuclear import of RNA-binding protein HuR

Wang, W., Yang, X., Kawai, T., De Silanes, I. L., Mazan-Mamczarz, K., Chen, P., Yuh, M. C., Quensel, C., Köhler, M. & Gorospe, M., Nov 12 2004, In : Journal of Biological Chemistry. 279, 46, p. 48376-48388 13 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear RNA
Acetylation
Phosphorylation
AMP-Activated Protein Kinases
2002
42 Citations (Scopus)

Uncoupling Kapβ2 substrate dissociation and ran binding

Chook, Y. M., Jung, A., Rosen, M. K. & Blobel, G., Jun 4 2002, In : Biochemistry. 41, 22, p. 6955-6966 12 p.

Research output: Contribution to journalArticle

GTP Phosphohydrolases
Carrier Proteins
ran GTP-Binding Protein
Binding Sites
Digitonin
2001
363 Citations (Scopus)

Karyopherins and nuclear import

Yuh, M. C. & Blobel, G., Dec 1 2001, In : Current Opinion in Structural Biology. 11, 6, p. 703-715 13 p.

Research output: Contribution to journalArticle

Karyopherins
Cell Nucleus Active Transport
Nuclear Pore Complex Proteins
Nuclear Pore
Nuclear Localization Signals
1999
13 Citations (Scopus)

Pictures in cell biology structures of nuclear-transport components

Chook, Y. M., Cingolani, G., Conti, E., Stewart, M., Vetter, I. & Wittinghofer, A., Aug 1 1999, In : Trends in Cell Biology. 9, 8, p. 310-311 2 p.

Research output: Contribution to journalArticle

ran GTP-Binding Protein
Nuclear Pore Complex Proteins
Nuclear Pore
Cell Nucleus Active Transport
GTP Phosphohydrolases
272 Citations (Scopus)

Structure of the nuclear transport complex karyopherin-β2-Ran·GppNHp

Chook, Y. M. & Blobel, G., May 20 1999, In : Nature. 399, 6733, p. 230-237 8 p.

Research output: Contribution to journalArticle

Karyopherins
Cell Nucleus Active Transport
Guanosine Triphosphate
Nuclear Pore
GTP Phosphohydrolases
1998
19 Citations (Scopus)

Detection and use of pseudo-translation in determination of protein structures

Chook, Y. M., Lipscomb, W. N. & Hengming, K. E., Sep 1 1998, In : Acta Crystallographica Section D: Biological Crystallography. 54, 5, p. 822-827 6 p.

Research output: Contribution to journalArticle

Chorismate Mutase
Cyclophilins
Parity
proteins
symmetry
1997
63 Citations (Scopus)

High affinity binding of the pleckstrin homology domain of mSos1 to phosphatidylinositol (4,5)-bisphosphate

Kubiseski, T. J., Chook, Y. M., Parris, W. E., Rozakis-Adcock, M. & Pawson, T., 1997, In : Journal of Biological Chemistry. 272, 3, p. 1799-1804 6 p.

Research output: Contribution to journalArticle

Phosphatidylinositols
Phospholipids
Substitution reactions
ras Proteins
Inositol 1,4,5-Trisphosphate
1996
59 Citations (Scopus)

The Grb2-mSos1 complex binds phosphopeptides with higher affinity than Grb2

Chook, Y. M., 1996, In : Journal of Biological Chemistry. 271, 48, p. 30472-30478 7 p.

Research output: Contribution to journalArticle

Phosphopeptides
src Homology Domains
Epidermal Growth Factor Receptor
Epidermal Growth Factor
ras Proteins
1994
153 Citations (Scopus)
Chorismate Mutase
Bacillus subtilis
Amino Acids
Catalytic Domain
Water
1993
167 Citations (Scopus)
Chorismate Mutase
Bacillus subtilis
Catalytic Domain
Water
Enzymes