β-Catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state

Thomas D. Marose, Calli E. Merkel, Andrew P. McMahon, Thomas J. Carroll

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Differentiation is the process by which tissues/organs take on their final, physiologically functional form. This process is mediated in part by the silencing of embryonic genes and the activation of terminal, differentiation gene products. Mammalian kidney development is initiated when the Wolffian duct branches and invades the overlying metanephric mesenchyme. The newly formed epithelial bud, known as the ureteric bud, will continue to branch ultimately differentiating into the collecting duct system and ureter. Here, we show that Hoxb7-Cre mediated removal of β-catenin from the mouse Wolffian duct epithelium leads to the premature expression of gene products normally associated with the differentiated kidney collecting duct system including the water channel protein, Aquaporin-3 and the tight junction protein isoform, ZO-1α+. Mutant cells fail to maintain expression of some genes associated with embryonic development, including several mediators of branching morphogenesis, which subsequently leads to kidney aplasia or hypoplasia. Reciprocally, expression of a stabilized form of β-catenin appears to block differentiation of the collecting ducts. All of these defects occur in the absence of any effects on the adherens junctions. These data indicate a role for β-catenin in maintaining cells of the Wolffian ducts and the duct derived ureteric bud/collecting duct system in an undifferentiated or precursor state.

Original languageEnglish (US)
Pages (from-to)112-126
Number of pages15
JournalDevelopmental Biology
Volume314
Issue number1
DOIs
StatePublished - Feb 1 2008

Fingerprint

Wolffian Ducts
Catenins
Epithelium
Aquaporin 3
Collecting Kidney Tubules
Zonula Occludens-1 Protein
Kidney
Gene Expression
Adherens Junctions
Aquaporins
Mesoderm
Ureter
Morphogenesis
Transcriptional Activation
Embryonic Development
Protein Isoforms
Genes

Keywords

  • β-catenin
  • CAKUT
  • Differentiation
  • Kidney development
  • Metanephros
  • Precursor cell
  • Ureteric bud
  • Wnt
  • Wolffian duct

ASJC Scopus subject areas

  • Developmental Biology

Cite this

β-Catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state. / Marose, Thomas D.; Merkel, Calli E.; McMahon, Andrew P.; Carroll, Thomas J.

In: Developmental Biology, Vol. 314, No. 1, 01.02.2008, p. 112-126.

Research output: Contribution to journalArticle

Marose, Thomas D. ; Merkel, Calli E. ; McMahon, Andrew P. ; Carroll, Thomas J. / β-Catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state. In: Developmental Biology. 2008 ; Vol. 314, No. 1. pp. 112-126.
@article{bbafd9b4cff64e7fa8b737b8d28f8daf,
title = "β-Catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state",
abstract = "Differentiation is the process by which tissues/organs take on their final, physiologically functional form. This process is mediated in part by the silencing of embryonic genes and the activation of terminal, differentiation gene products. Mammalian kidney development is initiated when the Wolffian duct branches and invades the overlying metanephric mesenchyme. The newly formed epithelial bud, known as the ureteric bud, will continue to branch ultimately differentiating into the collecting duct system and ureter. Here, we show that Hoxb7-Cre mediated removal of β-catenin from the mouse Wolffian duct epithelium leads to the premature expression of gene products normally associated with the differentiated kidney collecting duct system including the water channel protein, Aquaporin-3 and the tight junction protein isoform, ZO-1α+. Mutant cells fail to maintain expression of some genes associated with embryonic development, including several mediators of branching morphogenesis, which subsequently leads to kidney aplasia or hypoplasia. Reciprocally, expression of a stabilized form of β-catenin appears to block differentiation of the collecting ducts. All of these defects occur in the absence of any effects on the adherens junctions. These data indicate a role for β-catenin in maintaining cells of the Wolffian ducts and the duct derived ureteric bud/collecting duct system in an undifferentiated or precursor state.",
keywords = "β-catenin, CAKUT, Differentiation, Kidney development, Metanephros, Precursor cell, Ureteric bud, Wnt, Wolffian duct",
author = "Marose, {Thomas D.} and Merkel, {Calli E.} and McMahon, {Andrew P.} and Carroll, {Thomas J.}",
year = "2008",
month = "2",
day = "1",
doi = "10.1016/j.ydbio.2007.11.016",
language = "English (US)",
volume = "314",
pages = "112--126",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - β-Catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state

AU - Marose, Thomas D.

AU - Merkel, Calli E.

AU - McMahon, Andrew P.

AU - Carroll, Thomas J.

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Differentiation is the process by which tissues/organs take on their final, physiologically functional form. This process is mediated in part by the silencing of embryonic genes and the activation of terminal, differentiation gene products. Mammalian kidney development is initiated when the Wolffian duct branches and invades the overlying metanephric mesenchyme. The newly formed epithelial bud, known as the ureteric bud, will continue to branch ultimately differentiating into the collecting duct system and ureter. Here, we show that Hoxb7-Cre mediated removal of β-catenin from the mouse Wolffian duct epithelium leads to the premature expression of gene products normally associated with the differentiated kidney collecting duct system including the water channel protein, Aquaporin-3 and the tight junction protein isoform, ZO-1α+. Mutant cells fail to maintain expression of some genes associated with embryonic development, including several mediators of branching morphogenesis, which subsequently leads to kidney aplasia or hypoplasia. Reciprocally, expression of a stabilized form of β-catenin appears to block differentiation of the collecting ducts. All of these defects occur in the absence of any effects on the adherens junctions. These data indicate a role for β-catenin in maintaining cells of the Wolffian ducts and the duct derived ureteric bud/collecting duct system in an undifferentiated or precursor state.

AB - Differentiation is the process by which tissues/organs take on their final, physiologically functional form. This process is mediated in part by the silencing of embryonic genes and the activation of terminal, differentiation gene products. Mammalian kidney development is initiated when the Wolffian duct branches and invades the overlying metanephric mesenchyme. The newly formed epithelial bud, known as the ureteric bud, will continue to branch ultimately differentiating into the collecting duct system and ureter. Here, we show that Hoxb7-Cre mediated removal of β-catenin from the mouse Wolffian duct epithelium leads to the premature expression of gene products normally associated with the differentiated kidney collecting duct system including the water channel protein, Aquaporin-3 and the tight junction protein isoform, ZO-1α+. Mutant cells fail to maintain expression of some genes associated with embryonic development, including several mediators of branching morphogenesis, which subsequently leads to kidney aplasia or hypoplasia. Reciprocally, expression of a stabilized form of β-catenin appears to block differentiation of the collecting ducts. All of these defects occur in the absence of any effects on the adherens junctions. These data indicate a role for β-catenin in maintaining cells of the Wolffian ducts and the duct derived ureteric bud/collecting duct system in an undifferentiated or precursor state.

KW - β-catenin

KW - CAKUT

KW - Differentiation

KW - Kidney development

KW - Metanephros

KW - Precursor cell

KW - Ureteric bud

KW - Wnt

KW - Wolffian duct

UR - http://www.scopus.com/inward/record.url?scp=38349104769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349104769&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2007.11.016

DO - 10.1016/j.ydbio.2007.11.016

M3 - Article

VL - 314

SP - 112

EP - 126

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -