β3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons

Karine Pozo, Lorenzo A. Cingolani, Silvia Bassani, Fanny Laurent, Maria Passafaro, Yukiko Goda

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The integrins are transmembrane receptors for ECM proteins, and they regulate various cellular functions in the central nervous system. In hippocampal neurons, the β3 integrin subtype is required for homeostatic synaptic scaling of AMPA receptors (AMPARs) induced by chronic activity deprivation. The surface level of β3 integrin in postsynaptic neurons directly correlates with synaptic strength and the abundance of synaptic GluA2 AMPAR subunit. Although these observations suggest a functional link between β3 integrin and AMPAR, little is known about the mechanistic basis for the connection. Here we investigate the nature of β3 integrin and AMPAR interaction underlying the β3 integrin-dependent control of synaptic AMPAR expression and thus synaptic strength. We show that β3 integrin and GluA2 subunit form a complex in mouse brain that involves the direct binding between their cytoplasmic domains. In contrast, β3 integrin associates with GluA1 AMPAR subunit only weakly, and, in a heterologous expression system, the interaction requires the coexpression of GluA2. Surprisingly, in hippocampal pyramidal neurons, expressing β3 integrin mutants with either increased or decreased affinity for extracellular ligands has no differential effects in elevating excitatory synaptic currents and surface GluA2 levels compared withWT β3 integrin. Our findings identify an integrin family member, β3, as a direct interactor of an AMPAR subunit and provide molecular insights into how this cell-adhesion protein regulates the composition of cell-surface AMPARs.

Original languageEnglish (US)
Pages (from-to)1323-1328
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number4
DOIs
StatePublished - Jan 24 2012

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AMPA Receptors
Integrins
Neurons
Neurotransmitter Receptor
Pyramidal Cells
Cell Surface Receptors
Cell Adhesion
Proteins
Central Nervous System
Ligands

Keywords

  • Excitatory synaptic transmission
  • Extracellular matrix
  • Homeostatic synaptic plasticity

ASJC Scopus subject areas

  • General

Cite this

β3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. / Pozo, Karine; Cingolani, Lorenzo A.; Bassani, Silvia; Laurent, Fanny; Passafaro, Maria; Goda, Yukiko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 4, 24.01.2012, p. 1323-1328.

Research output: Contribution to journalArticle

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AU - Passafaro, Maria

AU - Goda, Yukiko

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