β2-Glycoprotein I-dependent alterations in membrane properties

Anh Tuyet Lee, Krishnakumar Balasubramanian, Alan J. Schroit

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

β2-Glycoprotein I (β2GP1), a 50 kDa serum glycoprotein, binds anionic phospholipids and plays a role in phosphatidylserine (PS)-dependent coagulation and apoptotic processes. To characterize the molecular consequences that occur to target membranes upon binding of β2GP1, the interaction between β2GP1 and PS-containing vesicles was investigated by fluorescent spectroscopy. Membranes containing pyrene-labeled lipid showed that binding of β2GP1 induced a decrease in excimer/monomor ratios (E/M) of the target membrane. Although these membrane alterations occurred in isotonic buffer, the effects were greater in low ionic strength buffer and were coincident to membrane precipitation. In contrast, increases in membrane polarization were only seen in low ionic strength buffer. Analysis of β2GP1 binding kinetics by resonance energy transfer between fluorescein-labeled β2GP1 and rhodamine-containing PS vesicles revealed a two-component process: (1) a primary and rapid binding via the C-terminus that occurred < 2 s in both isotonic and low ionic strength buffers, and (2) a sequential binding of the N-terminus that was ~100-fold slower in low ionic strength solution. Taken together, these data suggest that β2GP1 alters the fluidity and membrane polarization of its target membrane, which in low ionic strength buffer is of sufficient magnitude to induce precipitation. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)475-484
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1509
Issue number1-2
DOIs
StatePublished - Dec 20 2000

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Glycoproteins
Osmolar Concentration
Membranes
Ionic strength
Buffers
Phosphatidylserines
Polarization
Membrane Fluidity
Rhodamines
Energy Transfer
Fluidity
Fluorescein
Coagulation
Phospholipids
Spectrum Analysis
Energy transfer
Lipids
Spectroscopy
Kinetics
Serum

Keywords

  • β-Glycoprotein 1
  • Apolipoprotein H
  • Phosphatidylserine
  • Small unilamellar vesicle

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

β2-Glycoprotein I-dependent alterations in membrane properties. / Lee, Anh Tuyet; Balasubramanian, Krishnakumar; Schroit, Alan J.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1509, No. 1-2, 20.12.2000, p. 475-484.

Research output: Contribution to journalArticle

Lee, Anh Tuyet ; Balasubramanian, Krishnakumar ; Schroit, Alan J. / β2-Glycoprotein I-dependent alterations in membrane properties. In: Biochimica et Biophysica Acta - Biomembranes. 2000 ; Vol. 1509, No. 1-2. pp. 475-484.
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