γδ T cells are required for maximal expression of allergic conjunctivitis

Nancy J. Reyes, Elizabeth Mayhew, Peter W. Chen, Jerry Y. Niederkorn

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

PURPOSE. To determine the function of γδ T cells in early- and late-phase responses in allergic conjunctivitis. METHODS. Wild-type (WT) C57BL/6 and γδ T cell- deficient (TCR-δ-/-) mice were immunized intraperitoneally and challenged topically for 7 consecutive days with short ragweed pollen. Natural killer T (NKT) and γδ T cell- double-deficient mice were generated by treating TCR-δ-/- mice with anti- CD1d antibody. Allergic conjunctivitis was evaluated clinically, and the late-phase response was assessed by histopathology. Cytokine profiles were evaluated by ELISA. The afferent and efferent arms of allergic conjunctivitis were assessed by adoptive transfer of CD4+ T cells from WT or TCR-δ-/- mice into naive TCR-δ-/- or WT mice. RESULTS. TCR-δ-/- mice had decreased clinical manifestations of allergic conjunctivitis compared with WT mice. TCR-δ-/- mice had decreased eosinophilic infiltration compared with WT mice. TCR-δ-/- mice produced less Th2-associated cytokines interleukin (IL)-4, -5, and -13 compared with WT mice. Clinical manifestations of allergic conjunctivitis were lowest in NKT cell- depleted TCR-δ-/- mice. However, late-phase allergic conjunctivitis in NKT cell- depleted, TCR-δ-/- mice was the same as TCR-δ-/- mice. Adoptive transfer of CD4+ T cells revealed that γδ T cells are needed for the afferent and efferent arms of allergic conjunctivitis. CONCLUSIONS. γδ T cells are needed for full expression of both the clinical manifestations and the late phase of allergic conjunctivitis. Thus, γδ T cells have an important impact in the expression of allergic conjunctivitis and are a potential therapeutic target in the management of allergic diseases of the ocular surface.

Original languageEnglish (US)
Pages (from-to)2211-2216
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number5
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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