We have used highly specific affinity-purified antibodies against mouse κ- and λ-chains in conjunction with both immunofluorescence analysis on the FACS and enzymatic radioiodination of murine splenocytes and bone marrow cells. Our results indicate that the κ/λ ratio of cell surface immunoglobulin is constant during the ontogenetic development of B cells, i.e., a shift in the κ/λ ratio does not occur during B cell maturation. Our results suggest that the events controlling κ and λ expression on murine B cells are decided very early in ontogeny, before significant antigenic selection.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1981|
ASJC Scopus subject areas
- Immunology and Allergy