20-HETE and F2-isoprostanes in the metabolic syndrome: the effect of weight reduction

I. Jung Tsai, Kevin D. Croft, Trevor A. Mori, J R Falck, Lawrence J. Beilin, Ian B. Puddey, Anne E. Barden

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P450 metabolite of arachidonic acid that regulates vascular function and sodium homeostasis. Studies showing an association between 20-HETE excretion, raised BMI, and oxidative stress suggest that 20-HETE may be important in the development of cardiovascular disease in the metabolic syndrome (MetS). We investigated whether 20-HETE and F2-isoprostanes (markers of oxidative stress) were altered in the MetS before and after weight reduction. A case-controlled comparison of 30 participants with the MetS and matched controls showed that plasma and urinary 20-HETE and F2-isoprostanes were significantly elevated in the MetS group. There was a significant gender × group interaction such that women with the MetS had higher urinary 20-HETE and F2-isoprostanes compared to controls (p < 0.0001). In a randomized controlled trial, 42 participants with the MetS were assigned to 16 weeks of weight maintenance or a 12-week weight-loss program followed by 4 weeks weight stabilization. Relative to the weight-maintenance group, a 4-kg loss in weight resulted in a 2-mm Hg fall in blood pressure (BP) but did not alter urinary or plasma 20-HETE or F2-isoprostanes. 20-HETE and oxidative stress may be important mediators of cardiovascular disease risk in the MetS. Although a 4% reduction in body weight reduced BP, there were no changes in plasma or urinary 20-HETE or F2-isoprostanes.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalFree Radical Biology and Medicine
Volume46
Issue number2
DOIs
StatePublished - Jan 15 2009

Keywords

  • F-isoprostanes
  • Free radicals
  • Metabolic syndrome
  • Plasma and urinary 20-HETE
  • Weight reduction

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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