3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region

Gunamani Sithanandam, Farida Latif, Fuh Mei Duh, Ricardo Bernal, Ute Smola, Hua Li, Igor Kuzmin, Viktor Wixler, Laura Geil, Sadeep Shrestha, Patricia A. Lloyd, Scott Bader, Yoshitaka Sekido, Kenneth D. Tartof, Vladimir I. Kashuba, Eugene R. Zabarovsky, Michael Dean, George Klein, Michael I. Lerman, John D. MinnaUlf R. Rapp, Rando Allikmets

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

NotI linking clones, localized to the human chromosome 3p21.3 region and homozygously deleted in small cell lung cancer cell lines NCI-H740 and NCI- H1450, were used to search for a putative tumor suppressor gene(s). One of these clones, NL1G210, detected a 2.5-kb mRNA in all examined human tissues, expression being especially high in the heart and skeletal muscle. Two overlapping cDNA clones containing the entire open reading frame were isolated from a human heart cDNA library and fully characterized. Computer analysis and a search of the GenBank database revealed high sequence identity of the product of this gene to serine-threonine kinases, especially to mitogen-activated protein kinase-activated protein kinase 2, a recently described substrate of mitogen-activated kinases. Sequence identity was 72% at the nucleotide level and 75% at the amino acid level, strongly suggesting that this protein is a serine-threonine kinase. Here we demonstrate that the new gene, referred to as 3pK (for chromosome 3p kinase), in fact encodes a mitogen-activated protein kinase-regulated protein serine-threonine kinase with a novel substrate specificity.

Original languageEnglish (US)
Pages (from-to)868-876
Number of pages9
JournalMolecular and cellular biology
Volume16
Issue number3
DOIs
StatePublished - Mar 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Sithanandam, G., Latif, F., Duh, F. M., Bernal, R., Smola, U., Li, H., Kuzmin, I., Wixler, V., Geil, L., Shrestha, S., Lloyd, P. A., Bader, S., Sekido, Y., Tartof, K. D., Kashuba, V. I., Zabarovsky, E. R., Dean, M., Klein, G., Lerman, M. I., ... Allikmets, R. (1996). 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Molecular and cellular biology, 16(3), 868-876. https://doi.org/10.1128/MCB.16.3.868