3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region

Gunamani Sithanandam, Farida Latif, Fuh Mei Duh, Ricardo Bernal, Ute Smola, Hua Li, Igor Kuzmin, Viktor Wixler, Laura Geil, Sadeep Shrestha, Patricia A. Lloyd, Scott Bader, Yoshitaka Sekido, Kenneth D. Tartof, Vladimir I. Kashuba, Eugene R. Zabarovsky, Michael Dean, George Klein, Michael I. Lerman, John D. MinnaUlf R. Rapp, Rando Allikmets

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

NotI linking clones, localized to the human chromosome 3p21.3 region and homozygously deleted in small cell lung cancer cell lines NCI-H740 and NCI- H1450, were used to search for a putative tumor suppressor gene(s). One of these clones, NL1G210, detected a 2.5-kb mRNA in all examined human tissues, expression being especially high in the heart and skeletal muscle. Two overlapping cDNA clones containing the entire open reading frame were isolated from a human heart cDNA library and fully characterized. Computer analysis and a search of the GenBank database revealed high sequence identity of the product of this gene to serine-threonine kinases, especially to mitogen-activated protein kinase-activated protein kinase 2, a recently described substrate of mitogen-activated kinases. Sequence identity was 72% at the nucleotide level and 75% at the amino acid level, strongly suggesting that this protein is a serine-threonine kinase. Here we demonstrate that the new gene, referred to as 3pK (for chromosome 3p kinase), in fact encodes a mitogen-activated protein kinase-regulated protein serine-threonine kinase with a novel substrate specificity.

Original languageEnglish (US)
Pages (from-to)868-876
Number of pages9
JournalMolecular and Cellular Biology
Volume16
Issue number3
StatePublished - Mar 1996

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Protein-Serine-Threonine Kinases
Small Cell Lung Carcinoma
Mitogen-Activated Protein Kinases
Tumor Suppressor Genes
Protein Kinases
Phosphotransferases
Clone Cells
Chromosomes
Nucleic Acid Databases
Human Chromosomes
Substrate Specificity
Gene Library
Mitogens
Open Reading Frames
Genes
Myocardium
Skeletal Muscle
Nucleotides
Complementary DNA
Databases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Sithanandam, G., Latif, F., Duh, F. M., Bernal, R., Smola, U., Li, H., ... Allikmets, R. (1996). 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Molecular and Cellular Biology, 16(3), 868-876.

3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. / Sithanandam, Gunamani; Latif, Farida; Duh, Fuh Mei; Bernal, Ricardo; Smola, Ute; Li, Hua; Kuzmin, Igor; Wixler, Viktor; Geil, Laura; Shrestha, Sadeep; Lloyd, Patricia A.; Bader, Scott; Sekido, Yoshitaka; Tartof, Kenneth D.; Kashuba, Vladimir I.; Zabarovsky, Eugene R.; Dean, Michael; Klein, George; Lerman, Michael I.; Minna, John D.; Rapp, Ulf R.; Allikmets, Rando.

In: Molecular and Cellular Biology, Vol. 16, No. 3, 03.1996, p. 868-876.

Research output: Contribution to journalArticle

Sithanandam, G, Latif, F, Duh, FM, Bernal, R, Smola, U, Li, H, Kuzmin, I, Wixler, V, Geil, L, Shrestha, S, Lloyd, PA, Bader, S, Sekido, Y, Tartof, KD, Kashuba, VI, Zabarovsky, ER, Dean, M, Klein, G, Lerman, MI, Minna, JD, Rapp, UR & Allikmets, R 1996, '3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region', Molecular and Cellular Biology, vol. 16, no. 3, pp. 868-876.
Sithanandam, Gunamani ; Latif, Farida ; Duh, Fuh Mei ; Bernal, Ricardo ; Smola, Ute ; Li, Hua ; Kuzmin, Igor ; Wixler, Viktor ; Geil, Laura ; Shrestha, Sadeep ; Lloyd, Patricia A. ; Bader, Scott ; Sekido, Yoshitaka ; Tartof, Kenneth D. ; Kashuba, Vladimir I. ; Zabarovsky, Eugene R. ; Dean, Michael ; Klein, George ; Lerman, Michael I. ; Minna, John D. ; Rapp, Ulf R. ; Allikmets, Rando. / 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 3. pp. 868-876.
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AU - Bernal, Ricardo

AU - Smola, Ute

AU - Li, Hua

AU - Kuzmin, Igor

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AU - Shrestha, Sadeep

AU - Lloyd, Patricia A.

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AU - Sekido, Yoshitaka

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AU - Kashuba, Vladimir I.

AU - Zabarovsky, Eugene R.

AU - Dean, Michael

AU - Klein, George

AU - Lerman, Michael I.

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AU - Allikmets, Rando

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